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- PDB-3mkt: Structure of a Cation-bound Multidrug and Toxin Compound Extrusio... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3mkt | ||||||
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Title | Structure of a Cation-bound Multidrug and Toxin Compound Extrusion (MATE) transporter | ||||||
![]() | Multi antimicrobial extrusion protein (Na(+)/drug antiporter) MATE-like MDR efflux pump | ||||||
![]() | TRANSPORT PROTEIN / MATE / multidrug transporter / cation-bound | ||||||
Function / homology | : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | He, X. / Szewczyk, P. / Karyakin, A. / Evin, M. / Hong, W.-X. / Zhang, Q. / Chang, G. | ||||||
![]() | ![]() Title: Structure of a cation-bound multidrug and toxic compound extrusion transporter. Authors: He, X. / Szewczyk, P. / Karyakin, A. / Evin, M. / Hong, W.X. / Zhang, Q. / Chang, G. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR DETERMINED |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 167.1 KB | Display | ![]() |
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PDB format | ![]() | 134.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.8 KB | Display | ![]() |
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Full document | ![]() | 667.4 KB | Display | |
Data in XML | ![]() | 65.8 KB | Display | |
Data in CIF | ![]() | 87.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 49761.133 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.83 Å3/Da / Density % sol: 67.86 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 7.6 Details: 50mM Tris-HCl pH 7.2-8.6, 87 mM (NH4)2SO4, and 16-24% polyethylene glycol 250 dimethyl ether in D2O, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 13, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0093 Å / Relative weight: 1 |
Reflection | Resolution: 3.65→19.77 Å / Num. obs: 15364 / Biso Wilson estimate: 30.5 Å2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 51.3938 Å2 / ksol: 0.25 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 133.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.65→19.77 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTR | ||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3.65→3.88 Å / Rfactor Rfree error: 0.038 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: protein_rep.param / Topol file: protein.top |