+Open data
-Basic information
Entry | Database: PDB / ID: 3mk9 | ||||||
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Title | Stabilized Ricin Immunogen 1-33/44-198 | ||||||
Components | Ricin | ||||||
Keywords | HYDROLASE / disulfide bond / NUCLEOTIDE-BINDING / PLANT DEFENSE / PROTEIN SYNTHESIS INHIBITOR / TOXIN / IMMUNOGEN | ||||||
Function / homology | Function and homology information rRNA N-glycosylase / rRNA N-glycosylase activity / AMP binding / defense response / toxin activity / carbohydrate binding / killing of cells of another organism / negative regulation of translation Similarity search - Function | ||||||
Biological species | Ricinus communis (castor bean) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.08 Å | ||||||
Authors | Legler, P.M. / Compton, J.R. / Millard, C.B. | ||||||
Citation | Journal: Proteins / Year: 2011 Title: Introduction of a disulfide bond leads to stabilization and crystallization of a ricin immunogen. Authors: Compton, J.R. / Legler, P.M. / Clingan, B.V. / Olson, M.A. / Millard, C.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3mk9.cif.gz | 49.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3mk9.ent.gz | 34.1 KB | Display | PDB format |
PDBx/mmJSON format | 3mk9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mk/3mk9 ftp://data.pdbj.org/pub/pdb/validation_reports/mk/3mk9 | HTTPS FTP |
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-Related structure data
Related structure data | 3lc9C 1ifsS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 21445.240 Da / Num. of mol.: 1 / Mutation: V49C, E99C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ricinus communis (castor bean) / Production host: Escherichia coli (E. coli) / Strain (production host): BL-21(DE3) / References: UniProt: P02879, rRNA N-glycosylase |
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#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.85 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 6.4 Details: Protein Buffer: 50mM MES pH 6.4, 200mM NaCl, 2mM BME, Crystallization Solution: 0.17M Ammonium Sulfate, 25.5% w/v PEG 4000, 15% v/v Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: BRUKER AXS MICROSTAR / Wavelength: 1.54 Å |
Detector | Type: BRUKER SMART 6000 / Detector: CCD / Date: Feb 21, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.08→58.07 Å / Num. all: 11284 / Num. obs: 10903 / % possible obs: 96.6 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 9.61 % / Rsym value: 0.054 / Net I/σ(I): 20.18 |
Reflection shell | Resolution: 2.08→2.17 Å / Redundancy: 3.01 % / Mean I/σ(I) obs: 3.88 / % possible all: 77.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1IFS Resolution: 2.08→58.03 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.916 / Cross valid method: THROUGHOUT / ESU R: 0.252 / ESU R Free: 0.188 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.692 Å2
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Refinement step | Cycle: LAST / Resolution: 2.08→58.03 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.08→2.134 Å / Total num. of bins used: 20
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