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- PDB-3ly6: Crystal structure of human transglutaminase 2 complex with adenos... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ly6 | ||||||
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Title | Crystal structure of human transglutaminase 2 complex with adenosine 5' Triphosphate | ||||||
![]() | Protein-glutamine gamma-glutamyltransferase 2 | ||||||
![]() | TRANSFERASE / transglutaminase / Acyltransferase / Diabetes mellitus / Disease mutation / Metal-binding / Phosphoprotein | ||||||
Function / homology | ![]() histone serotonyltransferase activity / histone dopaminyltransferase activity / peptide noradrenalinyltransferase activity / peptide histaminyltransferase activity / cellular response to serotonin / regulation of apoptotic cell clearance / protein deamination / protein-glutamine glutaminase activity / protein-glutamine glutaminase / protein-glutamine gamma-glutamyltransferase ...histone serotonyltransferase activity / histone dopaminyltransferase activity / peptide noradrenalinyltransferase activity / peptide histaminyltransferase activity / cellular response to serotonin / regulation of apoptotic cell clearance / protein deamination / protein-glutamine glutaminase activity / protein-glutamine glutaminase / protein-glutamine gamma-glutamyltransferase / positive regulation of mitochondrial calcium ion concentration / salivary gland cavitation / protein-glutamine gamma-glutamyltransferase activity / negative regulation of endoplasmic reticulum calcium ion concentration / dopamine secretion / peptide cross-linking / branching involved in salivary gland morphogenesis / cellular response to dopamine / positive regulation of small GTPase mediated signal transduction / apoptotic cell clearance / Hydrolases; Acting on peptide bonds (peptidases) / cellular response to cocaine / positive regulation of neurogenesis / positive regulation of cell adhesion / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / extracellular matrix / positive regulation of GTPase activity / bone development / protein homooligomerization / nucleosome / phospholipase C-activating G protein-coupled receptor signaling pathway / peptidase activity / gene expression / regulation of apoptotic process / collagen-containing extracellular matrix / positive regulation of apoptotic process / focal adhesion / calcium ion binding / chromatin / GTP binding / perinuclear region of cytoplasm / endoplasmic reticulum / mitochondrion / proteolysis / extracellular exosome / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Han, B.G. / Lee, B.I. | ||||||
![]() | ![]() Title: Crystal structure of human transglutaminase 2 in complex with adenosine triphosphate Authors: Han, B.G. / Cho, J.W. / Cho, Y.D. / Jeong, K.C. / Kim, S.Y. / Lee, B.I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 366.6 KB | Display | ![]() |
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PDB format | ![]() | 305.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 83.8 KB | Display | |
Data in CIF | ![]() | 111.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1kv3S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 78718.062 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P21980, protein-glutamine gamma-glutamyltransferase #2: Chemical | Sequence details | THIS SEQUENCE IS FROM GENBANK, AAA63261. SEE REFERENCE 1 IN UNP DATABASE, P21980. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.63 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.6 Details: 50MM MES, 200MM NACL, 50MM MGCL2, 6-8% PEG3350,5MM DTT, 20% GLYCEROL, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 4r / Detector: CCD / Date: Jan 10, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.14→50 Å / Num. all: 42242 / Num. obs: 41905 / % possible obs: 99.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 3.14→3.26 Å / % possible all: 98.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1KV3 Resolution: 3.14→30 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 3.14→30 Å
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