+Open data
-Basic information
Entry | Database: PDB / ID: 3jtn | ||||||
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Title | Crystal Structure of the c-terminal domain of YpbH | ||||||
Components | Adapter protein mecA 2 | ||||||
Keywords | PROTEIN BINDING / YpbH / adaptor protein / Competence / Sporulation | ||||||
Function / homology | Function and homology information negative regulation of establishment of competence for transformation / negative regulation of sporulation resulting in formation of a cellular spore / establishment of competence for transformation / sporulation resulting in formation of a cellular spore / protein-macromolecule adaptor activity Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / PHASER / Resolution: 2.09 Å | ||||||
Authors | Wang, F. / Mei, Z. / Qi, Y. / Yan, C. / Wang, J. / Shi, Y. | ||||||
Citation | Journal: To be Published Title: Crystal Structure of the MecA degradation tag Authors: Wang, F. / Mei, Z. / Qi, Y. / Yan, C. / Xiang, S. / Zhou, Z. / Hu, Q. / Wang, J. / Shi, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3jtn.cif.gz | 82.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3jtn.ent.gz | 64.8 KB | Display | PDB format |
PDBx/mmJSON format | 3jtn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3jtn_validation.pdf.gz | 444.6 KB | Display | wwPDB validaton report |
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Full document | 3jtn_full_validation.pdf.gz | 450.8 KB | Display | |
Data in XML | 3jtn_validation.xml.gz | 10.8 KB | Display | |
Data in CIF | 3jtn_validation.cif.gz | 13.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jt/3jtn ftp://data.pdbj.org/pub/pdb/validation_reports/jt/3jtn | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 10182.252 Da / Num. of mol.: 2 / Fragment: c-terminal domain, UNP residues 104-194 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: ypbH / Plasmid: pGEX-6p-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P50734 #2: Chemical | ChemComp-IOD / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.29 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 20% PEG 3350,0.3M calcium chloride, 0.1M Bis-Tris pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS HTC / Detector: IMAGE PLATE / Date: Jul 22, 2009 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.09→34.363 Å / Num. all: 17571 / Num. obs: 17571 / % possible obs: 94.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.9 % / Biso Wilson estimate: 30.99 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 9.09 |
Reflection shell | Resolution: 2.09→2.21 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.732 / Mean I/σ(I) obs: 2.6 / Num. unique all: 17571 / % possible all: 88 |
-Processing
Software |
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Refinement | Method to determine structure: PHASER / Resolution: 2.09→34.363 Å / Occupancy max: 1 / Occupancy min: 0.21 / FOM work R set: 0.698 / SU ML: 0.35 / σ(F): 0.03 / Phase error: 36.01 / Stereochemistry target values: ML Details: The file contains friedel pairs in the _refln.pdbx_F_plus and _refln.pdbx_F_minus columns.
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 37.434 Å2 / ksol: 0.388 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 226.24 Å2 / Biso mean: 43.401 Å2 / Biso min: 15.09 Å2
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Refinement step | Cycle: LAST / Resolution: 2.09→34.363 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 6
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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