IN ORDER TO DETERMINE THE CRYSTAL STRUCTURE OF THIS PROTEIN BY SAD PHASING USING SE-MET SIGNAL, LEU ...IN ORDER TO DETERMINE THE CRYSTAL STRUCTURE OF THIS PROTEIN BY SAD PHASING USING SE-MET SIGNAL, LEU 115 WAS MUTATED TO MET. RESIDUE 124 IS THR, NOT PRO BASED ON THE CURRENT CRYSTAL STRUCTURE, FOR THE ELECTRON DENSITY FOR THR IS UNAMBIGUOUS. THE PRESENT CRYSTAL STRUCTURE WAS OBTAINED BY CLONING A GENE FROM THE STRAIN DSM2067 (DEUTSCHE COLLECTION) OR ATCC 43000, WHOSE CLOSEST MATCH IS THE MMARC7_1297 GENE FROM METHANOCOCCUS MARIPALUDIS C7.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.26 Å3/Da / 溶媒含有率: 45.51 %
結晶化
温度: 291 K / 手法: マイクロバッチ法, under oil / pH: 5.5 詳細: Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1 M Bis-Tris (pH 5.5), 25% PEG3350, and 0.2 M Lithium sulfate, microbatch, under oil, temperature 291K