Mass: 18.015 Da / Num. of mol.: 669 / Source method: isolated from a natural source / Formula: H2O
Sequence details
IN ORDER TO DETERMINE THE CRYSTAL STRUCTURE OF THIS PROTEIN BY SAD PHASING USING SE-MET SIGNAL, LEU ...IN ORDER TO DETERMINE THE CRYSTAL STRUCTURE OF THIS PROTEIN BY SAD PHASING USING SE-MET SIGNAL, LEU 115 WAS MUTATED TO MET. RESIDUE 124 IS THR, NOT PRO BASED ON THE CURRENT CRYSTAL STRUCTURE, FOR THE ELECTRON DENSITY FOR THR IS UNAMBIGUOUS. THE PRESENT CRYSTAL STRUCTURE WAS OBTAINED BY CLONING A GENE FROM THE STRAIN DSM2067 (DEUTSCHE COLLECTION) OR ATCC 43000, WHOSE CLOSEST MATCH IS THE MMARC7_1297 GENE FROM METHANOCOCCUS MARIPALUDIS C7.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.26 Å3/Da / Density % sol: 45.51 %
Crystal grow
Temperature: 291 K / Method: microbatch, under oil / pH: 5.5 Details: Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1 M Bis-Tris (pH 5.5), 25% PEG3350, and 0.2 M Lithium sulfate, microbatch, under oil, temperature 291K
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