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Yorodumi- PDB-3htj: Crystal structure of multidrug binding protein EbrR complexed wit... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3htj | ||||||
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Title | Crystal structure of multidrug binding protein EbrR complexed with ethidium | ||||||
Components | EbrA repressor | ||||||
Keywords | DNA BINDING PROTEIN / TetR family / multidrug resistance / Multidrug binding protein / DNA-binding / Transcription / Transcription regulation | ||||||
Function / homology | Function and homology information transcription cis-regulatory region binding / DNA-binding transcription factor activity Similarity search - Function | ||||||
Biological species | Streptomyces lividans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Dong, J. / Ni, L. / Schumacher, M. / Brennan, R. | ||||||
Citation | Journal: To be Published Title: Structural plasticity is key to multiple ligand binding by the multidrug binding regulator EbrR Authors: Dong, J. / Ni, L. / Schumacher, M. / Brennan, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3htj.cif.gz | 80.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3htj.ent.gz | 59.6 KB | Display | PDB format |
PDBx/mmJSON format | 3htj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3htj_validation.pdf.gz | 1001.3 KB | Display | wwPDB validaton report |
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Full document | 3htj_full_validation.pdf.gz | 1019.6 KB | Display | |
Data in XML | 3htj_validation.xml.gz | 12.1 KB | Display | |
Data in CIF | 3htj_validation.cif.gz | 16.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ht/3htj ftp://data.pdbj.org/pub/pdb/validation_reports/ht/3htj | HTTPS FTP |
-Related structure data
Related structure data | 3htaSC 3hthC 3htiC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 23266.268 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces lividans (bacteria) / Strain: TK64 / Gene: ebrR / Plasmid: pET15b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q79SH7 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 58.17 % |
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Crystal grow | Temperature: 295 K / pH: 9 Details: 1.2 M Lithium sulfate, 0.1 M Tris-HCl, 0.01 M Nickel chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1 |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 27, 2008 |
Radiation | Monochromator: SI(111) DOUBLE FLAT CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→74.33 Å / Num. obs: 14305 / % possible obs: 98.1 % / Redundancy: 2.2 % / Rmerge(I) obs: 0.053 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 3HTA Resolution: 2.7→74.33 Å / Isotropic thermal model: ANISOTROPIC / Cross valid method: THROUGHOUT
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.7→74.33 Å
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Refine LS restraints |
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