ソフトウェア | 名称 | バージョン | 分類 |
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ADSC | Quantumデータ収集 | HKL-3000 | | 位相決定 | REFMAC | 5.5.0054精密化 | HKL-3000 | | データ削減 | DENZO | | データ削減 | SCALEPACK | | データスケーリング | | |
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精密化 | 構造決定の手法: 単波長異常分散 / 解像度: 3.25→47.3 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.883 / SU B: 59.755 / SU ML: 0.445 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R Free: 0.576 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE
| Rfactor | 反射数 | %反射 | Selection details |
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Rfree | 0.294 | 354 | 4.7 % | RANDOM |
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Rwork | 0.221 | - | - | - |
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obs | 0.225 | 7566 | 99.5 % | - |
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK |
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原子変位パラメータ | Biso mean: 26.9 Å2
| Baniso -1 | Baniso -2 | Baniso -3 |
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1- | 0.04 Å2 | 0 Å2 | 0 Å2 |
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2- | - | 0.04 Å2 | 0 Å2 |
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3- | - | - | -0.09 Å2 |
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精密化ステップ | サイクル: LAST / 解像度: 3.25→47.3 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 2516 | 0 | 10 | 10 | 2536 |
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拘束条件 | Refine-ID | タイプ | Dev ideal | Dev ideal target | 数 |
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X-RAY DIFFRACTION | r_bond_refined_d0.011 | 0.022 | 2552 | X-RAY DIFFRACTION | r_bond_other_d | | | X-RAY DIFFRACTION | r_angle_refined_deg1.471 | 1.989 | 3452 | X-RAY DIFFRACTION | r_angle_other_deg | | | X-RAY DIFFRACTION | r_dihedral_angle_1_deg6.765 | 5 | 335 | X-RAY DIFFRACTION | r_dihedral_angle_2_deg34.644 | 23.263 | 95 | X-RAY DIFFRACTION | r_dihedral_angle_3_deg21.072 | 15 | 428 | X-RAY DIFFRACTION | r_dihedral_angle_4_deg19.368 | 15 | 19 | X-RAY DIFFRACTION | r_chiral_restr0.096 | 0.2 | 419 | X-RAY DIFFRACTION | r_gen_planes_refined0.006 | 0.02 | 1853 | X-RAY DIFFRACTION | r_gen_planes_other | | | X-RAY DIFFRACTION | r_nbd_refined | | | X-RAY DIFFRACTION | r_nbd_other | | | X-RAY DIFFRACTION | r_nbtor_refined | | | X-RAY DIFFRACTION | r_nbtor_other | | | X-RAY DIFFRACTION | r_xyhbond_nbd_refined | | | X-RAY DIFFRACTION | r_xyhbond_nbd_other | | | X-RAY DIFFRACTION | r_metal_ion_refined | | | X-RAY DIFFRACTION | r_metal_ion_other | | | X-RAY DIFFRACTION | r_symmetry_vdw_refined | | | X-RAY DIFFRACTION | r_symmetry_vdw_other | | | X-RAY DIFFRACTION | r_symmetry_hbond_refined | | | X-RAY DIFFRACTION | r_symmetry_hbond_other | | | X-RAY DIFFRACTION | r_symmetry_metal_ion_refined | | | X-RAY DIFFRACTION | r_symmetry_metal_ion_other | | | X-RAY DIFFRACTION | r_mcbond_it0.409 | 1.5 | 1678 | X-RAY DIFFRACTION | r_mcbond_other | | | X-RAY DIFFRACTION | r_mcangle_it0.788 | 2 | 2663 | X-RAY DIFFRACTION | r_scbond_it1.182 | 3 | 874 | X-RAY DIFFRACTION | r_scangle_it2.119 | 4.5 | 789 | X-RAY DIFFRACTION | r_rigid_bond_restr | | | X-RAY DIFFRACTION | r_sphericity_free | | | X-RAY DIFFRACTION | r_sphericity_bonded | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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LS精密化 シェル | 解像度: 3.25→3.33 Å / Total num. of bins used: 20
| Rfactor | 反射数 | %反射 |
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Rfree | 0.295 | 24 | - |
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Rwork | 0.262 | 508 | - |
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obs | - | - | 98.89 % |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 1.8235 | 1.2936 | 1.4686 | 1.3142 | 0.5011 | 1.9691 | -0.0749 | 0.0866 | 0.1892 | 0.1869 | 0.0178 | 0.1931 | -0.3739 | 0.2847 | 0.0571 | 0.3941 | 0.0262 | 0.0208 | 0.4225 | 0.0214 | 0.3621 | -22.0617 | 17.8608 | -9.3857 | 2 | 3.8046 | 3.0349 | 1.7154 | 7.0438 | -0.1266 | 2.1575 | -0.3068 | 0.0553 | 0.5516 | 0.2358 | 0.1893 | -0.1243 | -0.8379 | 0.5795 | 0.1175 | 0.5493 | -0.241 | 0.0774 | 0.5611 | -0.1054 | 0.4475 | -16.9874 | 25.3351 | -9.4128 | 3 | 2.9951 | 0.9996 | 1.2339 | 1.0912 | 2.3752 | 6.8572 | -0.0038 | -0.2124 | 0.4606 | 0.1025 | 0.0795 | -0.0256 | -0.6217 | 0.6253 | -0.0756 | 0.6799 | -0.1154 | -0.0593 | 0.5021 | -0.0388 | 0.4297 | -17.3413 | 24.4193 | -2.1043 | 4 | 7.0335 | 0.8187 | -0.4757 | 6.7795 | 2.3544 | 4.4434 | 0.2135 | -0.5232 | -0.2524 | 1.0241 | 0.1934 | -1.0093 | 0.6125 | 1.4579 | -0.4069 | 0.6212 | 0.1283 | -0.2388 | 0.7948 | -0.0369 | 0.414 | -8.9651 | 10.7089 | 3.6257 | 5 | 4.7173 | -2.6951 | 0.9443 | 4.6162 | 0.1036 | 0.4062 | 0.383 | 1.3712 | 0.0841 | -0.809 | -0.7913 | 0.269 | -0.118 | 0.0481 | 0.4084 | 0.5028 | 0.1121 | -0.1082 | 0.9087 | -0.1579 | 0.5875 | -5.9957 | 3.0754 | 0.0706 | 6 | 3.2099 | -0.7594 | 1.8666 | 5.5696 | 1.8228 | 7.1611 | 0.3868 | -0.082 | -0.6 | 0.8744 | 0.1206 | -0.7063 | 1.2163 | 0.8871 | -0.5074 | 0.9358 | 0.1896 | -0.2204 | 0.6375 | 0.0891 | 0.5387 | -15.6477 | 0.9414 | 0.9496 | 7 | 4.3153 | -1.5779 | -1.4066 | 2.7074 | -2.4656 | 4.7915 | -0.2754 | -0.6374 | 0.0062 | 0.3939 | 0.2269 | -0.1045 | 0.046 | 0.2977 | 0.0485 | 0.8319 | 0.0658 | -0.134 | 0.5543 | 0.0274 | 0.3409 | -20.6037 | 11.0375 | 5.438 | 8 | 0.4592 | 0.2293 | 1.0269 | 4.4949 | 0.2609 | 4.5643 | -0.0325 | -0.4512 | 0.306 | 1.0084 | -0.047 | 0.2719 | -0.6019 | -0.2705 | 0.0795 | 0.8267 | -0.0633 | 0.0748 | 0.8741 | -0.2888 | 0.6205 | -22.4089 | 24.1953 | 8.1429 | 9 | 3.6167 | 0.1242 | 0.4324 | 0.3001 | 1.4434 | 7.1189 | 0.129 | -0.1501 | 0.1307 | 0.0472 | -0.0104 | -0.018 | -0.0358 | 0.3842 | -0.1186 | 0.4832 | -0.0192 | 0.0336 | 0.4022 | 0.0683 | 0.3282 | -22.6652 | 15.4558 | -3.7989 | 10 | 4.9395 | -4.2798 | 2.2204 | 6.5832 | 1.2459 | 5.5983 | -0.0934 | -0.2879 | -0.5761 | 1.075 | 0.0081 | 0.2937 | 0.8674 | -0.3687 | 0.0853 | 0.5831 | -0.0271 | 0.0335 | 0.4254 | 0.0811 | 0.4299 | -28.2874 | 11.2243 | 0.0917 | 11 | 1.1739 | 0.8726 | 1.4716 | 3.2694 | 0.9607 | 4.8587 | 0.1405 | -0.1182 | 0.3207 | 0.2915 | -0.0933 | 0.4181 | -0.6491 | -0.4489 | -0.0472 | 0.3532 | 0.0384 | 0.0482 | 0.3886 | 0.0409 | 0.3109 | -27.3677 | 17.0014 | -7.217 | 12 | 7.4343 | 1.4681 | 2.3884 | 8.461 | 1.7521 | 8.5381 | -0.3351 | 0.7316 | 0.0505 | -0.4201 | 0.6637 | -0.4826 | -0.6602 | 1.4847 | -0.3286 | 0.3354 | -0.1437 | 0.0716 | 0.8181 | -0.0385 | 0.3938 | -12.6116 | 14.2603 | -23.9447 | 13 | 9.5451 | 5.5548 | 3.4759 | 4.2708 | 2.5504 | 2.7049 | -0.1127 | 0.4081 | -0.1003 | 0.1738 | 0.6746 | -0.9806 | -0.5844 | 1.6751 | -0.5619 | 0.6547 | -0.4704 | -0.0393 | 1.7605 | -0.3427 | 0.9092 | -3.306 | 15.9735 | -18.839 | 14 | 9.6853 | 3.6092 | 2.7695 | 6.6445 | 2.9465 | 4.2127 | -0.0389 | 0.7789 | -0.8068 | -0.0337 | 0.7275 | -1.8135 | 0.2193 | 2.3467 | -0.6886 | 0.506 | 0.1401 | 0.0197 | 1.5287 | -0.192 | 0.8213 | -7.0171 | 5.4854 | -21.4401 | 15 | 13.6779 | 4.0967 | -3.3378 | 11.915 | -2.4697 | 13.4347 | 0.1791 | 0.2414 | -1.3595 | 0.5029 | 0.4679 | -0.7941 | 0.964 | 1.6262 | -0.647 | 0.5589 | 0.1869 | -0.0644 | 0.7797 | -0.1011 | 0.6445 | -13.7322 | 2.7224 | -16.147 | 16 | 7.7681 | -1.0785 | 1.3257 | 6.4468 | 0.7592 | 7.2318 | 0.0535 | -0.1397 | -0.5709 | 0.549 | 0.1133 | -0.1224 | 0.723 | 0.4535 | -0.1668 | 0.464 | 0.0401 | 0.0071 | 0.4771 | 0.0442 | 0.4248 | -23.6431 | 6.522 | -12.1595 | 17 | 4.2261 | 3.203 | 0.2967 | 8.7311 | -0.4584 | 5.3253 | -0.041 | -0.0459 | 0.7574 | 0.1868 | -0.0719 | 0.5758 | -0.8694 | -0.2116 | 0.1128 | 0.4606 | 0.0577 | -0.0116 | 0.4477 | 0.0759 | 0.4929 | -29.4126 | 18.9799 | -14.352 | 18 | 2.1389 | 0.5157 | 1.628 | 1.2444 | 0.9251 | 8.3438 | -0.6815 | 0.8336 | 1.1284 | -0.912 | 0.6709 | -0.1483 | -2.104 | 1.749 | 0.0106 | 1.1272 | -0.4728 | -0.0005 | 0.9669 | 0.1529 | 0.8231 | -17.3634 | 25.6028 | -22.6425 | 19 | 6.761 | 0.5531 | -1.369 | 2.55 | 1.5498 | 6.2574 | -0.4399 | 0.6489 | 0.8764 | -0.8064 | 0.3109 | 0.204 | -1.5688 | 0.681 | 0.129 | 0.8619 | -0.1944 | -0.0758 | 0.4678 | 0.175 | 0.5942 | -21.1937 | 30.0926 | -19.5578 | 20 | 2.8078 | 1.3191 | 0.5556 | 4.4516 | 1.7493 | 4.9615 | -0.2154 | -0.3685 | 1.0199 | 0.0901 | -0.2071 | 1.021 | -1.4282 | -0.4233 | 0.4225 | 0.8368 | 0.0975 | -0.0427 | 0.4579 | 0.0088 | 0.8036 | -29.353 | 30.0805 | -10.6172 | 21 | 1.6035 | -0.6668 | -4.0412 | 0.3977 | 1.5209 | 15.1259 | 0.1151 | 0.2372 | -0.0109 | -0.2429 | -0.0758 | 0.0752 | -0.7167 | 0.6334 | -0.0393 | 0.4277 | -0.1341 | -0.0606 | 0.6025 | 0.0652 | 0.2705 | -28.9633 | 8.788 | -35.1214 | 22 | 4.6499 | 0.2091 | 2.9273 | 1.6412 | 1.6037 | 7.673 | -0.2417 | 0.4546 | 0.4136 | -0.2988 | -0.1343 | 0.7066 | -0.3361 | -0.8249 | 0.376 | 0.2973 | 0.0308 | -0.0366 | 0.5455 | 0.0619 | 0.5079 | -38.578 | 11.3855 | -23.3418 | 23 | 4.7046 | 1.6474 | 0.1648 | 4.3865 | 2.0413 | 6.323 | -0.3928 | 0.5257 | 0.5785 | -0.6527 | 0.0869 | 0.7004 | -1.0142 | -0.6102 | 0.3059 | 0.5273 | 0.0206 | -0.2487 | 0.7001 | 0.1714 | 0.4755 | -39.5749 | 15.477 | -35.6434 | 24 | 3.4265 | -0.0559 | 1.4144 | 4.0969 | 3.9133 | 4.3703 | -0.1424 | 0.4017 | -0.0202 | -0.222 | -0.2799 | 0.3426 | -0.3097 | -0.2442 | 0.4223 | 0.2686 | -0.1036 | -0.1419 | 0.6932 | 0.04 | 0.3115 | -38.5015 | 5.1149 | -35.4622 | 25 | 6.3009 | -0.9328 | 2.7191 | 6.1144 | -2.7206 | 4.8455 | -0.0728 | 0.5905 | -0.2687 | 0.0165 | -0.0819 | 0.6761 | 0.6036 | -1.0693 | 0.1547 | 0.4833 | -0.2413 | -0.0264 | 0.7952 | -0.1225 | 0.5788 | -42.6679 | -4.8236 | -31.104 | 26 | 1.0499 | 0.9815 | -1.2445 | 0.9956 | -0.8867 | 4.2131 | -0.6354 | 0.5861 | -0.0905 | -0.6459 | 0.7589 | -0.2795 | 0.5405 | 0.435 | -0.1235 | 0.6628 | -0.4011 | 0.0425 | 0.8789 | -0.1889 | 0.657 | -46.0383 | -13.086 | -27.2513 | 27 | 5.1761 | -0.2089 | -0.4333 | 1.0061 | 2.263 | 7.4105 | 0.2046 | -0.0209 | -0.42 | 0.5897 | -0.2358 | 0.435 | 1.3325 | -1.0688 | 0.0312 | 0.7577 | -0.273 | 0.0703 | 0.6363 | 0.0055 | 0.7305 | -40.865 | -8.6699 | -20.9563 | 28 | 5.2236 | 1.1126 | 1.8003 | 5.0505 | 0.7743 | 3.8431 | 0.1179 | 0.238 | -0.8654 | 0.4235 | 0.0941 | -0.176 | 1.2995 | 0.001 | -0.2119 | 0.6656 | -0.0745 | -0.02 | 0.4393 | -0.0945 | 0.5563 | -32.1524 | -10.3211 | -25.6551 | 29 | 3.9451 | 1.7702 | 0.5971 | 3.909 | -0.0025 | 8.6309 | -0.1534 | 1.1692 | -0.382 | -0.6531 | 0.2443 | 0.2452 | 0.6876 | -0.1929 | -0.091 | 0.4505 | -0.1251 | -0.0283 | 0.6662 | -0.1393 | 0.3913 | -33.7882 | -4.5011 | -36.7482 | 30 | 2.5657 | 1.9031 | 1.7831 | 3.7914 | -0.6994 | 4.5897 | -0.273 | 0.8802 | 0.027 | -0.7037 | 0.2724 | 0.2524 | 0.2344 | 0.0937 | 0.0006 | 0.4376 | -0.022 | -0.0327 | 0.8214 | -0.0106 | 0.3903 | -31.6304 | 3.4831 | -35.3243 | 31 | 3.5536 | -0.138 | -1.9146 | 4.2152 | 3.3308 | 9.691 | -0.1735 | 0.829 | -0.7959 | -0.1119 | 0.4165 | -0.2066 | 0.7291 | 0.4401 | -0.243 | 0.3996 | 0.0018 | 0.0053 | 0.6294 | -0.1091 | 0.4109 | -25.1359 | -1.4593 | -31.0438 | 32 | 2.0283 | 2.3459 | 0.6877 | 4.5038 | -1.5364 | 4.7386 | -0.3225 | 0.818 | -0.0257 | -0.6169 | 0.4809 | -0.2857 | -0.4686 | 0.6426 | -0.1585 | 0.4816 | -0.018 | 0.1331 | 0.8155 | 0.0309 | 0.4336 | -24.8108 | 6.6837 | -33.7641 | 33 | 4.2826 | 0.2643 | 1.2607 | 3.0498 | 1.2843 | 5.1596 | -0.1671 | -0.1636 | 0.4271 | 0.0868 | -0.1601 | 0.7274 | -0.3826 | -0.8257 | 0.3272 | 0.3491 | 0.0926 | 0.0242 | 0.5094 | 0.0334 | 0.551 | -37.7536 | 15.7511 | -15.1999 | 34 | 3.6312 | -4.0306 | 3.3624 | 6.6354 | -1.0331 | 7.3054 | -0.1291 | -0.2303 | 0.3657 | -0.24 | -0.438 | 0.4486 | -0.4302 | -1.2784 | 0.5671 | 0.3615 | 0.0773 | 0.0132 | 0.8183 | -0.043 | 1.0689 | -46.7373 | 14.4937 | -14.5236 | 35 | 1.2169 | 1.0744 | 0.7682 | 1.0046 | 1.0435 | 6.6554 | 0.2429 | -0.5424 | 0.4901 | 0.3783 | -0.5978 | 0.5763 | 0.5688 | -1.8226 | 0.3549 | 0.464 | -0.1424 | 0.295 | 0.8659 | -0.0498 | 0.8005 | -45.702 | 6.2719 | -8.5269 | 36 | 14.3805 | 3.7835 | -5.2037 | 15.2214 | -2.4275 | 11.9804 | 0.1805 | -0.53 | 0.1883 | 0.6084 | -0.1958 | 0.5835 | 0.6255 | -0.8076 | 0.0153 | 0.4605 | -0.1103 | 0.1302 | 0.58 | 0.0063 | 0.5008 | -39.7664 | 3.3082 | -10.7921 | 37 | 3.5465 | -4.3692 | 0.6662 | 6.5039 | 0.3562 | 1.3993 | 0.0821 | 0.0523 | -0.6936 | 0.4561 | -0.1558 | 0.9023 | 0.3834 | -0.3995 | 0.0737 | 0.4761 | -0.1035 | 0.0399 | 0.6133 | 0.0449 | 0.4689 | -34.0528 | 0.469 | -15.3212 | 38 | 6.05 | 0.5714 | 0.4432 | 3.2556 | 1.1036 | 5.8534 | -0.2427 | 0.3819 | 0.286 | -0.1388 | 0.2165 | 0.2187 | -0.3832 | -0.0272 | 0.0262 | 0.3166 | -0.0173 | -0.0111 | 0.4212 | 0.0797 | 0.3281 | -28.5432 | 12.8589 | -22.3366 | 39 | 3.9284 | 0.1179 | -0.7856 | 2.8198 | 1.2595 | 5.3092 | -0.568 | 0.6312 | 0.8965 | -0.8007 | 0.0024 | 0.7407 | -1.3221 | -0.5108 | 0.5656 | 0.912 | 0.1411 | -0.2552 | 0.7315 | 0.2089 | 0.8729 | -37.447 | 24.6058 | -27.7978 | 40 | 8.0609 | 0.7535 | -8.1181 | 1.2248 | 0.8114 | 10.3316 | 0.0297 | 0.887 | 0.5021 | -0.9105 | 0.6284 | 0.1237 | -1.4638 | 0.2312 | -0.6581 | 1.0003 | -0.292 | -0.0333 | 1.0163 | 0.2057 | 0.5444 | -27.344 | 19.655 | -35.1548 |
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精密化 TLSグループ | ID | Refine-ID | Refine TLS-ID | Auth asym-ID | Auth seq-ID |
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1 | X-RAY DIFFRACTION | 1 | A9 - 20 | 2 | X-RAY DIFFRACTION | 2 | A21 - 29 | 3 | X-RAY DIFFRACTION | 3 | A30 - 39 | 4 | X-RAY DIFFRACTION | 4 | A40 - 48 | 5 | X-RAY DIFFRACTION | 5 | A49 - 56 | 6 | X-RAY DIFFRACTION | 6 | A57 - 70 | 7 | X-RAY DIFFRACTION | 7 | A71 - 78 | 8 | X-RAY DIFFRACTION | 8 | A79 - 86 | 9 | X-RAY DIFFRACTION | 9 | A87 - 97 | 10 | X-RAY DIFFRACTION | 10 | A98 - 103 | 11 | X-RAY DIFFRACTION | 11 | A104 - 119 | 12 | X-RAY DIFFRACTION | 12 | A120 - 125 | 13 | X-RAY DIFFRACTION | 13 | A126 - 130 | 14 | X-RAY DIFFRACTION | 14 | A135 - 142 | 15 | X-RAY DIFFRACTION | 15 | A143 - 147 | 16 | X-RAY DIFFRACTION | 16 | A148 - 158 | 17 | X-RAY DIFFRACTION | 17 | A159 - 165 | 18 | X-RAY DIFFRACTION | 18 | A166 - 170 | 19 | X-RAY DIFFRACTION | 19 | A171 - 176 | 20 | X-RAY DIFFRACTION | 20 | A177 - 185 | 21 | X-RAY DIFFRACTION | 21 | B9 - 13 | 22 | X-RAY DIFFRACTION | 22 | B14 - 23 | 23 | X-RAY DIFFRACTION | 23 | B24 - 31 | 24 | X-RAY DIFFRACTION | 24 | B32 - 39 | 25 | X-RAY DIFFRACTION | 25 | B40 - 45 | 26 | X-RAY DIFFRACTION | 26 | B46 - 52 | 27 | X-RAY DIFFRACTION | 27 | B53 - 64 | 28 | X-RAY DIFFRACTION | 28 | B65 - 72 | 29 | X-RAY DIFFRACTION | 29 | B73 - 81 | 30 | X-RAY DIFFRACTION | 30 | B82 - 97 | 31 | X-RAY DIFFRACTION | 31 | B98 - 104 | 32 | X-RAY DIFFRACTION | 32 | B105 - 114 | 33 | X-RAY DIFFRACTION | 33 | B115 - 122 | 34 | X-RAY DIFFRACTION | 34 | B123 - 128 | 35 | X-RAY DIFFRACTION | 35 | B137 - 142 | 36 | X-RAY DIFFRACTION | 36 | B143 - 146 | 37 | X-RAY DIFFRACTION | 37 | B147 - 152 | 38 | X-RAY DIFFRACTION | 38 | B153 - 167 | 39 | X-RAY DIFFRACTION | 39 | B168 - 178 | 40 | X-RAY DIFFRACTION | 40 | B179 - 185 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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