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- PDB-3gti: D71G/E101G/M234L mutant in organophosphorus hydrolase from Deinoc... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3gti | ||||||
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Title | D71G/E101G/M234L mutant in organophosphorus hydrolase from Deinococcus radiodurans | ||||||
![]() | Organophosphorus hydrolase | ||||||
![]() | HYDROLASE / alpha-beta barrel / amidohydrolase / mutant | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hawwa, R. / Larsen, S. / Ratia, K. / Mesecar, A. | ||||||
![]() | ![]() Title: Structure-based and random mutagenesis approaches increase the organophosphate-degrading activity of a phosphotriesterase homologue from Deinococcus radiodurans. Authors: Hawwa, R. / Larsen, S.D. / Ratia, K. / Mesecar, A.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 80.5 KB | Display | ![]() |
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PDB format | ![]() | 62.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 432.9 KB | Display | ![]() |
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Full document | ![]() | 442.6 KB | Display | |
Data in XML | ![]() | 18.6 KB | Display | |
Data in CIF | ![]() | 27.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3gtfC ![]() 3gthC ![]() 3gtxC ![]() 3gu1C ![]() 3gu2C ![]() 3gu9C ![]() 3htwC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 36295.785 Da / Num. of mol.: 1 / Mutation: D71G, E101G, M234L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: DR_0930 / Production host: ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.04 Å3/Da / Density % sol: 59.49 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1 M Bis-Tris, pH 6.5, 20% PEG MME 5000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Feb 14, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.42→20 Å / Num. obs: 17317 / % possible obs: 98.2 % |
Reflection shell | Resolution: 2.42→2.57 Å / % possible all: 98.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.42→20 Å
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Refine LS restraints |
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