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- PDB-3gi9: Crystal Structure of ApcT Transporter Bound to 7F11 Monoclonal Fa... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3gi9 | ||||||
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Title | Crystal Structure of ApcT Transporter Bound to 7F11 Monoclonal Fab Fragment | ||||||
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![]() | TRANSPORT PROTEIN / membrane protein / transporter / antibody / Cell membrane / Membrane / Transmembrane / Structural Genomics / PSI-2 / Protein Structure Initiative / New York Consortium on Membrane Protein Structure / NYCOMPS | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shaffer, P.L. / Goehring, A.S. / Shankaranarayanan, A. / Gouaux, E. / New York Consortium on Membrane Protein Structure (NYCOMPS) | ||||||
![]() | ![]() Title: Structure and mechanism of a na+-independent amino Acid transporter. Authors: Shaffer, P.L. / Goehring, A. / Shankaranarayanan, A. / Gouaux, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 173.8 KB | Display | ![]() |
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PDB format | ![]() | 142.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.5 KB | Display | ![]() |
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Full document | ![]() | 454.8 KB | Display | |
Data in XML | ![]() | 31.4 KB | Display | |
Data in CIF | ![]() | 44.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 24284.820 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Antibody | Mass: 23984.684 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Protein | Mass: 48330.219 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: MJ0609 / Plasmid: pET25b / Production host: ![]() ![]() |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.45 % |
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Crystal grow | Temperature: 293 K / Method: microbatch / pH: 5 Details: 0.1M sodium acetate, 0.1M rubidium chloride, 0.1M L-alanine, 40-48% PEG 400, pH 5.0, Microbatch, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 22, 2008 |
Radiation | Monochromator: Double Crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.48→50 Å / Num. obs: 36003 / % possible obs: 93.5 % / Observed criterion σ(I): -3 / Redundancy: 3.2 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 24.3 |
Reflection shell | Resolution: 2.48→2.59 Å / Redundancy: 2.1 % / Rmerge(I) obs: 0.302 / Mean I/σ(I) obs: 2.1 / % possible all: 70.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 51.006 Å2
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Refinement step | Cycle: LAST / Resolution: 2.48→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.48→2.541 Å / Total num. of bins used: 20
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