- PDB-3gan: Crystal structure of gene product from Arabidopsis thaliana At3g2... -
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Basic information
Entry
Database: PDB / ID: 3gan
Title
Crystal structure of gene product from Arabidopsis thaliana At3g22680 with bound suramin
Components
Uncharacterized protein At3g22680
Keywords
STRUCTURAL GENOMICS / UNKNOWN FUNCTION / suramin / Structural Genomics Functional Follow-Up Study / Protein Structure Initiative / PSI / Center for Eukaryotic Structural Genomics / CESG
Function / homology
Function and homology information
RNA polymerase V complex / gene silencing by siRNA-directed DNA methylation / siRNA processing / DNA binding / nucleoplasm / nucleus Similarity search - Function
RDM1 protein domain / Protein RDM1, plant / RDM1 superfamily / RNA-directed DNA methylation 1 / Four Helix Bundle (Hemerythrin (Met), subunit A) / Up-down Bundle / Mainly Alpha Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 57 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.44 Å3/Da / Density % sol: 49.5 %
Crystal grow
Temperature: 297 K / Method: vapor diffusion, hanging drop Details: Protein solution (10 mg/ml MSE Protein, 0.10 M NaCl, 0.0003 M TCEP, 0.010 M Suramin sodium salt, 0.01 M Hepes pH 7.0) mixed in a 1:1 ratio with the Well solution (1.1 M Sodium chloride, 1% ...Details: Protein solution (10 mg/ml MSE Protein, 0.10 M NaCl, 0.0003 M TCEP, 0.010 M Suramin sodium salt, 0.01 M Hepes pH 7.0) mixed in a 1:1 ratio with the Well solution (1.1 M Sodium chloride, 1% DMSO, 0.10 M BTP pH 7.0). Cryoprotected with 20% Ethylene glycol, 1.8 M Sodium chloride, 1% DMSO, 0.10 M BTP pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K
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