+Open data
-Basic information
Entry | Database: PDB / ID: 3fhc | ||||||
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Title | Crystal structure of human Dbp5 in complex with Nup214 | ||||||
Components |
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Keywords | TRANSPORT PROTEIN/HYDROLASE / DEAD-box helicase / mRNA export / Nucleoporin / beta propeller / RecA-like / RNA dependent ATPase / CAN / Ddx19 / Dead-box protein 19b / nuclear pore complex / Glycoprotein / mRNA transport / Nucleus / Phosphoprotein / Protein transport / Proto-oncogene / Translocation / Transport / ATP-binding / Helicase / Hydrolase / Membrane / Nucleotide-binding / RNA-binding / TRANSPORT PROTEIN-HYDROLASE COMPLEX | ||||||
Function / homology | Function and homology information cytoplasmic side of nuclear pore / HuR (ELAVL1) binds and stabilizes mRNA / regulation of nucleocytoplasmic transport / nuclear export signal receptor activity / Nuclear Pore Complex (NPC) Disassembly / Transport of Ribonucleoproteins into the Host Nucleus / Regulation of Glucokinase by Glucokinase Regulatory Protein / Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA ...cytoplasmic side of nuclear pore / HuR (ELAVL1) binds and stabilizes mRNA / regulation of nucleocytoplasmic transport / nuclear export signal receptor activity / Nuclear Pore Complex (NPC) Disassembly / Transport of Ribonucleoproteins into the Host Nucleus / Regulation of Glucokinase by Glucokinase Regulatory Protein / Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / NS1 Mediated Effects on Host Pathways / SUMOylation of SUMOylation proteins / Transport of Mature mRNA Derived from an Intronless Transcript / Rev-mediated nuclear export of HIV RNA / structural constituent of nuclear pore / SUMOylation of RNA binding proteins / Nuclear import of Rev protein / Transport of Mature mRNA derived from an Intron-Containing Transcript / NEP/NS2 Interacts with the Cellular Export Machinery / tRNA processing in the nucleus / RNA export from nucleus / nucleocytoplasmic transport / Viral Messenger RNA Synthesis / poly(A)+ mRNA export from nucleus / nuclear localization sequence binding / SUMOylation of ubiquitinylation proteins / Vpr-mediated nuclear import of PICs / SUMOylation of DNA replication proteins / Regulation of HSF1-mediated heat shock response / mRNA export from nucleus / SUMOylation of DNA damage response and repair proteins / nuclear pore / protein export from nucleus / SUMOylation of chromatin organization proteins / helicase activity / HCMV Late Events / Transcriptional regulation by small RNAs / ISG15 antiviral mechanism / cytoplasmic stress granule / HCMV Early Events / protein import into nucleus / nuclear envelope / snRNP Assembly / RNA helicase activity / regulation of cell cycle / RNA helicase / mRNA binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP hydrolysis activity / RNA binding / extracellular exosome / nucleoplasm / ATP binding / membrane / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | von Moeller, H. / Conti, E. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2009 Title: The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner Authors: von Moeller, H. / Basquin, C. / Conti, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3fhc.cif.gz | 130.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3fhc.ent.gz | 100.1 KB | Display | PDB format |
PDBx/mmJSON format | 3fhc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3fhc_validation.pdf.gz | 439.2 KB | Display | wwPDB validaton report |
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Full document | 3fhc_full_validation.pdf.gz | 452.4 KB | Display | |
Data in XML | 3fhc_validation.xml.gz | 24.4 KB | Display | |
Data in CIF | 3fhc_validation.cif.gz | 33.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fh/3fhc ftp://data.pdbj.org/pub/pdb/validation_reports/fh/3fhc | HTTPS FTP |
-Related structure data
Related structure data | 3fhtSC 2oitS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 44996.781 Da / Num. of mol.: 1 / Fragment: Nterminal beta propeller, UNP residues 1-405 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: Nup214 (Residues 1-405) / Plasmid: pETMC / Production host: Escherichia coli (E. coli) / Strain (production host): Bl21 / References: UniProt: P35658 |
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#2: Protein | Mass: 26525.752 Da / Num. of mol.: 1 / Fragment: Nterminal RecA-like domain, UNP residues 68-302 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: Dbp5 (Residues 68-302) / Plasmid: pETMC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 References: UniProt: Q9UMR2, Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.22 Å3/Da / Density % sol: 70.89 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 50mM MES pH 6.0, 900mM Na citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 0.99988 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 1, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99988 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→47.7 Å / Num. all: 30169 / Num. obs: 30169 / % possible obs: 100 % / Observed criterion σ(I): 3.3 / Redundancy: 14.13 % / Biso Wilson estimate: 45.8 Å2 / Rmerge(I) obs: 0.138 / Net I/σ(I): 11.59 |
Reflection shell | Resolution: 2.8→3 Å / Redundancy: 13.6 % / Rmerge(I) obs: 0.364 / Mean I/σ(I) obs: 3.31 / Num. unique all: 5545 / Rsym value: 0.36 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRIES 2OIT and 3FHT Resolution: 2.8→47.65 Å / Cor.coef. Fo:Fc: 0.908 / Cor.coef. Fo:Fc free: 0.866 / Occupancy max: 1 / Occupancy min: 0 / SU B: 13.023 / SU ML: 0.247 / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 3.31 / ESU R: 0.436 / ESU R Free: 0.302 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 85.99 Å2 / Biso mean: 42.137 Å2 / Biso min: 4.39 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→47.65 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.872 Å / Total num. of bins used: 20
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