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Yorodumi- PDB-3f8k: Crystal structure of protein acetyltransferase (PAT) from Sulfolo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3f8k | ||||||
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Title | Crystal structure of protein acetyltransferase (PAT) from Sulfolobus solfataricus | ||||||
Components | Protein acetyltransferase | ||||||
Keywords | TRANSFERASE / GCN5-related N-acetyltransferase | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Sulfolobus solfataricus P2 (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.84 Å | ||||||
Authors | Brent, M.M. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2009 Title: Structure and Biochemical Characterization of Protein Acetyltransferase from Sulfolobus solfataricus. Authors: Brent, M.M. / Iwata, A. / Carten, J. / Zhao, K. / Marmorstein, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3f8k.cif.gz | 42.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3f8k.ent.gz | 28.9 KB | Display | PDB format |
PDBx/mmJSON format | 3f8k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3f8k_validation.pdf.gz | 768.8 KB | Display | wwPDB validaton report |
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Full document | 3f8k_full_validation.pdf.gz | 771.7 KB | Display | |
Data in XML | 3f8k_validation.xml.gz | 8.9 KB | Display | |
Data in CIF | 3f8k_validation.cif.gz | 11.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f8/3f8k ftp://data.pdbj.org/pub/pdb/validation_reports/f8/3f8k | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18818.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus P2 (archaea) / Strain: DSM 1617 / JCM 11322 / P2 / Gene: SSO2813 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q97V23 |
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#2: Chemical | ChemComp-COA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 35.44 % Description: The structure factor file contains Friedel pairs |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 12% PEG 20000, 0.05M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 0.97932, 0.97945, 0.94932 | ||||||||||||
Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: Jul 3, 2006 / Details: Adjustable focus K-B pair Si plus Pt, Rh coatings | ||||||||||||
Radiation | Monochromator: Double crystal cryo-cooled Si(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.84→30 Å / Num. all: 23855 / Num. obs: 23521 / % possible obs: 98.6 % / Redundancy: 5.9 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 23.1 | ||||||||||||
Reflection shell | Resolution: 1.84→1.92 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.423 / Mean I/σ(I) obs: 2.5 / % possible all: 89.2 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.84→30 Å / Details: The Friedel pairs were used in phasing
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Solvent computation | Bsol: 68.0955 Å2 / ksol: 0.415774 e/Å3 | ||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.84→30 Å
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Refine LS restraints |
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