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- PDB-3eoq: The crystal structure of putative zinc protease beta-subunit from... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3eoq | ||||||
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Title | The crystal structure of putative zinc protease beta-subunit from Thermus thermophilus HB8 | ||||||
![]() | Putative zinc protease | ||||||
![]() | HYDROLASE / two similar domains of beta(2)-alpha(2)-beta(2)-alpha(5)-beta structure / Protease | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ohtsuka, J. / Ichihara, Y. / Ebihara, A. / Yokoyama, S. / Kuramitsu, S. / Nagata, K. / Tanokura, M. | ||||||
![]() | ![]() Title: Crystal structure of TTHA1264, a putative M16-family zinc peptidase from Thermus thermophilus HB8 that is homologous to the beta subunit of mitochondrial processing peptidase. Authors: Ohtsuka, J. / Ichihara, Y. / Ebihara, A. / Nagata, K. / Tanokura, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 166.8 KB | Display | ![]() |
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PDB format | ![]() | 141.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.3 KB | Display | ![]() |
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Full document | ![]() | 431.8 KB | Display | |
Data in XML | ![]() | 31.1 KB | Display | |
Data in CIF | ![]() | 44.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46058.750 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 49.11 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.15M DL-MALIC ACID, 22% PEG 3350, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 5, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. obs: 61353 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Biso Wilson estimate: 21.7 Å2 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 2.2 / % possible all: 98.2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.31 Å2
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Refinement step | Cycle: LAST / Resolution: 2.29→48.62 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.29→2.35 Å / Total num. of bins used: 20
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