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- PDB-3eiv: Crystal Structure of Single-stranded DNA-binding protein from Str... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3eiv | ||||||
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Title | Crystal Structure of Single-stranded DNA-binding protein from Streptomyces coelicolor | ||||||
![]() | Single-stranded DNA-binding protein 2 | ||||||
![]() | DNA BINDING PROTEIN / Single-stranded DNA-binding protein / Streptomyces Coelicolor / DNA damage / DNA repair / DNA replication / DNA-binding / Phosphoprotein | ||||||
Function / homology | ![]() positive regulation of helicase activity / nucleoid / single-stranded DNA binding / DNA replication Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() ![]() | ||||||
![]() | Luic, M. / Stefanic, Z. / Vujaklija, D. | ||||||
![]() | ![]() Title: Structure of the single-stranded DNA-binding protein from Streptomyces coelicolor. Authors: Stefanic, Z. / Vujaklija, D. / Luic, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 97.8 KB | Display | ![]() |
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PDB format | ![]() | 74 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 454 KB | Display | ![]() |
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Full document | ![]() | 461.3 KB | Display | |
Data in XML | ![]() | 20.2 KB | Display | |
Data in CIF | ![]() | 28.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1x3eS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | Biological unit is a tetramer. Biological unit is the same as asym. unit |
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Components
#1: Protein | Mass: 19925.531 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.83 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 50 mM TRIS-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Jul 21, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→32.2 Å / Num. all: 48150 / Num. obs: 31640 / % possible obs: 65.7 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 2.44 % / Biso Wilson estimate: 48.39 Å2 / Rmerge(I) obs: 0.038 / Rsym value: 0.0739 / Net I/σ(I): 13.373 |
Reflection shell | Resolution: 2.14→2.22 Å / Redundancy: 2.48 % / Rmerge(I) obs: 0.35 / Mean I/σ(I) obs: 1.5 / Num. measured all: 22946 / Num. unique all: 5720 / Rsym value: 0.41 / % possible all: 100 |
-Phasing
Phasing | Method: ![]() | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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Processing
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Refinement | Method to determine structure: ![]() ![]() Starting model: PDB ENTRY 1X3E Resolution: 2.141→31.182 Å / FOM work R set: 0.772 / Cross valid method: THROUGHOUT / σ(F): 2 / σ(I): 2 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 68.116 Å2 / ksol: 0.398 e/Å3 | ||||||||||||||||||||||||||||
Displacement parameters | Biso max: 90.21 Å2 / Biso mean: 46.76 Å2 / Biso min: 24.89 Å2
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Refinement step | Cycle: LAST / Resolution: 2.141→31.182 Å
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Refine LS restraints |
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