Synthesis of bile acids and bile salts via 24-hydroxycholesterol / 17-beta-ketosteroid reductase (NADPH) activity / RA biosynthesis pathway / steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor / Retinoid metabolism and transport / 3(or 17)alpha-hydroxysteroid dehydrogenase / steroid dehydrogenase activity / 17-beta-hydroxysteroid dehydrogenase (NADP+) activity / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / Synthesis of bile acids and bile salts via 27-hydroxycholesterol ...Synthesis of bile acids and bile salts via 24-hydroxycholesterol / 17-beta-ketosteroid reductase (NADPH) activity / RA biosynthesis pathway / steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor / Retinoid metabolism and transport / 3(or 17)alpha-hydroxysteroid dehydrogenase / steroid dehydrogenase activity / 17-beta-hydroxysteroid dehydrogenase (NADP+) activity / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / Synthesis of bile acids and bile salts via 27-hydroxycholesterol / Synthesis of Prostaglandins (PG) and Thromboxanes (TX) / Prednisone ADME / 5alpha-androstane-3beta,17beta-diol dehydrogenase activity / androsterone dehydrogenase activity / ketosteroid monooxygenase activity / aldo-keto reductase (NADPH) activity / lithocholic acid binding / steroid biosynthetic process / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / NADP+ binding / aldose reductase (NADPH) activity / steroid metabolic process / NADPH binding / steroid binding / cytosol Similarity search - Function
Aldo-keto reductase family 1 member C / NADP-dependent oxidoreductase domain / Aldo/keto reductase family signature 2. / Aldo/keto reductase, conserved site / Aldo-keto reductase / NADP-dependent oxidoreductase domain / Aldo/keto reductase family / NADP-dependent oxidoreductase domain superfamily / TIM Barrel / Alpha-Beta Barrel / Alpha Beta Similarity search - Domain/homology
BETA-MERCAPTOETHANOL / Chem-HXS / NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Aldo-keto reductase family 1 member C21 Similarity search - Component
Biological species
Mus musculus (house mouse)
Method
X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.1 Å
Mass: 18.015 Da / Num. of mol.: 664 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THERE ARE DIFFERENCES BETWEEN THE SEQRES AND THE SEQUENCE DATABASE. THIS IS A VARIANT AT THESE ...THERE ARE DIFFERENCES BETWEEN THE SEQRES AND THE SEQUENCE DATABASE. THIS IS A VARIANT AT THESE POSITIONS. THR221 IN MOLECULES A AND B MAKES CLOSE CONTACTS WITH THE COENZYME RESULTING IN A DEVIATION IN CHIRALITY.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.94 Å3/Da / Density % sol: 58.15 %
Crystal grow
Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1M Hepes, 10% PEG l6000, 5% 2-methyl-2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
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