THE DNA CAN PACK IN TWO ORIENTATIONS WHICH ARE NEARLY EQUIVALENT. ONLY 1/2 OF THE CRYSTALLIZED ...THE DNA CAN PACK IN TWO ORIENTATIONS WHICH ARE NEARLY EQUIVALENT. ONLY 1/2 OF THE CRYSTALLIZED SEQUENCE IS PRESENT IN THE COORDINATES. THE SECOND PART IS RELATED BY CRYSTAL SYMMETRY. THERE IS MIXED ELECTRON DENSITY OUTSIDE THE PALINDROMIC REGION BECAUSE THE TWOFOLD CRYSTALLOGRAPHIC AXIS COINCIDES WITH THE PSEUDO-TWOFOLD AXIS OF THE DNA. THE ENTRY IS PROCESSED AS 2 MODELS CORRESPONDING TO THE 2 ORIENTATIONS OF THE DNA MOLECULE.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 1.94 Å3/Da / 溶媒含有率: 36.53 %
結晶化
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.6 詳細: 19-22% polyethylene glycol 8000, 100 mM ammonium sulphate, 20mM magnesium chloride, 15% 2-methyl-2,4-pentanediol, 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH 7.6, VAPOR ...詳細: 19-22% polyethylene glycol 8000, 100 mM ammonium sulphate, 20mM magnesium chloride, 15% 2-methyl-2,4-pentanediol, 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K