- PDB-3cmv: Mechanism of homologous recombination from the RecA-ssDNA/dsDNA s... -
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データを開く
IDまたはキーワード:
読み込み中...
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基本情報
登録情報
データベース: PDB / ID: 3cmv
タイトル
Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
要素
Protein recA
キーワード
RECOMBINATION / homologous recombination
機能・相同性
機能・相同性情報
DNA polymerase V complex / homologous recombination / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / SOS response / ATP-dependent activity, acting on DNA / translesion synthesis / cell motility / single-stranded DNA binding ...DNA polymerase V complex / homologous recombination / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / SOS response / ATP-dependent activity, acting on DNA / translesion synthesis / cell motility / single-stranded DNA binding / DNA-binding transcription factor binding / DNA recombination / damaged DNA binding / DNA damage response / ATP hydrolysis activity / ATP binding / cytoplasm / cytosol 類似検索 - 分子機能
: / : / RecA C-terminal domain / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / recA signature. / DNA recombination and repair protein RecA / recA bacterial DNA recombination protein / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. ...: / : / RecA C-terminal domain / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / recA signature. / DNA recombination and repair protein RecA / recA bacterial DNA recombination protein / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / Protein RecA 類似検索 - 構成要素
pH: 6.5 詳細: RecA4 fusion protein mixed with 2 mM AMPPNP and 10 mM MgCl2, pH 7.5, followed by crystallization from 100 mM Bis-Tris-Cl, 7% (w/v) PEG 3350, 100 mM KSCN, 25% (v/v) ethylene glycol, pH 6.5.
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データ収集
放射光源
由来: シンクロトロン / サイト: APS / ビームライン: 24-ID-C
検出器
日付: 2007年8月1日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
相対比: 1
反射
解像度: 4.3→40 Å / Num. obs: 87622
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解析
ソフトウェア
名称: REFMAC / バージョン: 5.2.0019 / 分類: 精密化
精密化
構造決定の手法: 分子置換 / 解像度: 4.3→20 Å / Cor.coef. Fo:Fc: 0.929 / Cor.coef. Fo:Fc free: 0.92 / SU B: 150.646 / SU ML: 0.894 / 交差検証法: THROUGHOUT / ESU R Free: 1.051 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD
Rfactor
反射数
%反射
Selection details
Rfree
0.26144
1810
2 %
RANDOM
Rwork
0.24314
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obs
0.24351
87622
92.7 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: BABINET MODEL WITH MASK