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- PDB-3car: REDUCED STRUCTURE OF THE ACIDIC CYTOCHROME C3 FROM DESULFOVIBRIO ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3car | ||||||
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Title | REDUCED STRUCTURE OF THE ACIDIC CYTOCHROME C3 FROM DESULFOVIBRIO AFRICANUS | ||||||
![]() | CYTOCHROME C3 | ||||||
![]() | ELECTRON TRANSPORT / CYTOCHROME C3 / TETRAHEME / REDUCED FORM / DESULFOVIBRIO AFRICANUS | ||||||
Function / homology | ![]() anaerobic respiration / periplasmic space / electron transfer activity / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Norager, S. / Legrand, P. / Pieulle, L. / Hatchikian, C. / Roth, M. | ||||||
![]() | ![]() Title: Crystal structure of the oxidised and reduced acidic cytochrome c3from Desulfovibrio africanus. Authors: Norager, S. / Legrand, P. / Pieulle, L. / Hatchikian, C. / Roth, M. #1: ![]() Title: Crystallographic Studies of the Two Tetraheme Cytochromes C3 from Desulfovibrio Africanus Authors: Norager, S.C. #2: ![]() Title: Further Characterization of the Two Tetraheme Cytochromes C3 from Desulfovibrio Africanus: Nucleotide Sequences, Epr Spectroscopy and Biological Activity Authors: Magro, V. / Pieulle, L. / Forget, N. / Guigliarelli, B. / Petillot, Y. / Hatchikian, E.C. #3: ![]() Title: Biochemical Studies of the C-Type Cytochromes of the Sulfate Reducer Desulfovibrio Africanus. Characterization of Two Tetraheme Cytochromes C3 with Different Specificity Authors: Pieulle, L. / Haladjian, J. / Bonicel, J. / Hatchikian, E.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.9 KB | Display | ![]() |
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PDB format | ![]() | 31.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.8 MB | Display | ![]() |
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Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 10.6 KB | Display | |
Data in CIF | ![]() | 14 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 11278.314 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() | ||||||
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#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-HEM / #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.34 Å3/Da / Density % sol: 67 % | |||||||||||||||||||||||||
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Crystal grow | pH: 5.8 / Details: pH 5.80 | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: drop consists of equal volume of protein and reservoir solutions PH range low: 5.8 / PH range high: 5.6 | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 108.2 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: THOMSON/PRINCETON JNSJR / Detector: CCD AREA DETECTOR / Date: Jan 21, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0057 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→29 Å / Num. obs: 17501 / % possible obs: 97.9 % / Redundancy: 32.4 % / Biso Wilson estimate: 21.12 Å2 / Rsym value: 0.065 / Net I/σ(I): 8.6 |
Reflection shell | Resolution: 1.85→1.95 Å / Redundancy: 13.8 % / Mean I/σ(I) obs: 1.2 / Rsym value: 0.531 / % possible all: 87.4 |
Reflection | *PLUS Rmerge(I) obs: 0.065 |
Reflection shell | *PLUS % possible obs: 87.4 % / Rmerge(I) obs: 0.531 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: OXIDISED FORM OF THE SAME PROTEIN Resolution: 1.9→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.15 Details: RESIDUES WITH AN OCCUPANCY LOWER THAN 1.0, ARE REFINED WITH THE GIVEN OCCUPANCY. THIS OCCUPANCY HAS BEEN ESTABLISHED MANUALLY TO AVOID POSITIVE OR NEGATIVE PEAKS IN THE FOBS-FCALC DENSITY MAPS.
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Displacement parameters | Biso mean: 29.75 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Refine LS restraints |
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