regulation of transcription from RNA polymerase II promoter by galactose / kinase inhibitor activity / galactose metabolic process / negative regulation of phosphorylation / positive regulation of transcription from RNA polymerase II promoter by galactose / transcription repressor complex / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / nucleotide binding / negative regulation of transcription by RNA polymerase II ...regulation of transcription from RNA polymerase II promoter by galactose / kinase inhibitor activity / galactose metabolic process / negative regulation of phosphorylation / positive regulation of transcription from RNA polymerase II promoter by galactose / transcription repressor complex / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / nucleotide binding / negative regulation of transcription by RNA polymerase II / DNA binding / identical protein binding / nucleus / cytoplasm Similarity search - Function
Resolution: 2.85→50 Å / Num. all: 57929 / Num. obs: 54557 / % possible obs: 94.2 % / Observed criterion σ(I): -3 / Redundancy: 2.3 % / Biso Wilson estimate: 70.3 Å2 / Rmerge(I) obs: 0.076 / Net I/σ(I): 13.2
Reflection shell
Resolution: 2.85→2.95 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.372 / % possible all: 83
-
Processing
Software
Name
Version
Classification
REFMAC
5.2.0019
refinement
CBASS
datacollection
HKL-2000
datareduction
HKL-2000
datascaling
SHARP
phasing
PHENIX
refinement
Refinement
Method to determine structure: MIRAS / Resolution: 2.85→50 Å / Cor.coef. Fo:Fc: 0.904 / Cor.coef. Fo:Fc free: 0.838 / SU B: 48.543 / SU ML: 0.429 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.6 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.278
5497
10.1 %
RANDOM
Rwork
0.228
-
-
-
obs
-
49054
85.6 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK
Displacement parameters
Biso mean: 57.676 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-6.09 Å2
0 Å2
0.37 Å2
2-
-
-0.5 Å2
0 Å2
3-
-
-
6.7 Å2
Refinement step
Cycle: LAST / Resolution: 2.85→50 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
18389
0
0
0
18389
LS refinement shell
Resolution: 2.85→2.928 Å / Total num. of bins used: 20