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- PDB-3b2e: Crystal structure of S. cerevisiae Get3 in the open conformation ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3b2e | ||||||
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Title | Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1 cytosolic domain | ||||||
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![]() | HYDROLASE/TRANSPORT PROTEIN / Protein-protein interaction / receptor complex / HYDROLASE / TRANSPORT PROTEIN / ADP binding / Coild-coil / HYDROLASE-TRANSPORT PROTEIN complex | ||||||
Function / homology | ![]() GET complex / pheromone-dependent signal transduction involved in conjugation with cellular fusion / tail-anchored membrane protein insertion into ER membrane / Hydrolases; Acting on acid anhydrides / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / response to arsenic-containing substance / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / response to metal ion / mitophagy ...GET complex / pheromone-dependent signal transduction involved in conjugation with cellular fusion / tail-anchored membrane protein insertion into ER membrane / Hydrolases; Acting on acid anhydrides / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / response to arsenic-containing substance / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / response to metal ion / mitophagy / protein-membrane adaptor activity / protein folding chaperone / guanyl-nucleotide exchange factor activity / mitochondrial membrane / unfolded protein binding / response to heat / Golgi membrane / endoplasmic reticulum membrane / Golgi apparatus / endoplasmic reticulum / ATP hydrolysis activity / mitochondrion / ATP binding / identical protein binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kubota, K. / Yamagata, A. / Fukai, S. | ||||||
![]() | ![]() Title: Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces Authors: Kubota, K. / Yamagata, A. / Sato, Y. / Goto-Ito, S. / Fukai, S. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 309.9 KB | Display | ![]() |
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PDB format | ![]() | 250.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 65.4 KB | Display | |
Data in CIF | ![]() | 86.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3vlcC ![]() 3a36S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 40522.766 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: NRRL Y-53 / Plasmid: PETDuet / Production host: ![]() ![]() #2: Protein | Mass: 10064.349 Da / Num. of mol.: 4 / Fragment: Get1 cytosolic domain, UNP residues 20-103 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: GET1 / Plasmid: PETDuet / Production host: ![]() ![]() #3: Chemical | ChemComp-ADP / Sequence details | A SEQUENCE DATABASE REFERENCE FOR CHAIN A, B, C, D WHICH DERIVES FROM STRAIN NRRL Y-53 DOES NOT ...A SEQUENCE DATABASE REFERENCE FOR CHAIN A, B, C, D WHICH DERIVES FROM STRAIN NRRL Y-53 DOES NOT CURRENTLY EXIST. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.53 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 13.5% PEG3350, 0.18 M tri-sodium citrate, 9% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: May 21, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. obs: 44560 / % possible obs: 95 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.5 % / Rmerge(I) obs: 0.096 / Net I/σ(I): 19.1 |
Reflection shell | Resolution: 3→3.05 Å / Rmerge(I) obs: 0.469 / Mean I/σ(I) obs: 2 / % possible all: 87.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3A36 Resolution: 3→50 Å / σ(F): 0 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Refinement step | Cycle: LAST / Resolution: 3→50 Å
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Refine LS restraints |
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