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- PDB-3alm: Crystal structure of 2-methyl-3-hydroxypyridine-5-carboxylic acid... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3alm | ||||||
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Title | Crystal structure of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase, mutant C294A | ||||||
![]() | 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase | ||||||
![]() | OXIDOREDUCTASE / alpha/beta fold | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kobayashi, J. / Yoshida, H. / Yoshikane, Y. / Kamitori, S. / Yagi, T. | ||||||
![]() | ![]() Title: Crystal structure of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase Authors: Kobayashi, J. / Yoshida, H. / Yoshikane, Y. / Kamitori, S. / Yagi, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 164.9 KB | Display | ![]() |
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PDB format | ![]() | 129.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 975 KB | Display | ![]() |
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Full document | ![]() | 985.9 KB | Display | |
Data in XML | ![]() | 32.4 KB | Display | |
Data in CIF | ![]() | 46.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3aljC ![]() 3allC ![]() 4gf7C ![]() 4h2nC ![]() 4h2pC ![]() 4jy2C ![]() 4jy3C ![]() 3alh C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | BIOLOGICAL UNIT IS TETRAMER, BUT SPECIFIC INTERACTION FOR FORMING TETRAMER IS NOT OBSERVED. |
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Components
#1: Protein | Mass: 41766.426 Da / Num. of mol.: 2 / Mutation: C294A Source method: isolated from a genetically manipulated source Details: coexpression with chaperonine GroEL/ES / Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-GOL / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.21 Å3/Da / Density % sol: 61.74 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.1M Tris-HCl pH 8.5, 8% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: May 1, 2009 |
Radiation | Monochromator: Si-single crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.77→50 Å / Num. all: 101642 / Num. obs: 101642 / % possible obs: 99.3 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.079 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 1.77→1.8 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.38 / Num. unique all: 4789 / % possible all: 94.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3ALH ![]() 3alh Resolution: 1.77→35.42 Å / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Displacement parameters | Biso mean: 20.493 Å2
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Refinement step | Cycle: LAST / Resolution: 1.77→35.42 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.77→1.816 Å
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