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Open data
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Basic information
Entry | Database: PDB / ID: 3aai | ||||||
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Title | X-ray crystal structure of CsoR from Thermus thermophilus HB8 | ||||||
![]() | Copper homeostasis operon regulatory protein | ||||||
![]() | TRANSCRIPTION / All alpha proteins / 4-helix bundle | ||||||
Function / homology | ![]() DNA-binding transcription repressor activity / protein-DNA complex / transcription cis-regulatory region binding / negative regulation of DNA-templated transcription / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sakamoto, K. / Agari, Y. / Shinkai, A. / Kuramitsu, S. | ||||||
![]() | ![]() Title: Structural and functional characterization of the transcriptional repressor CsoR from Thermus thermophilus HB8 Authors: Sakamoto, K. / Agari, Y. / Agari, K. / Kuramitsu, S. / Shinkai, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.3 KB | Display | ![]() |
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PDB format | ![]() | 57 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 441.1 KB | Display | ![]() |
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Full document | ![]() | 447.4 KB | Display | |
Data in XML | ![]() | 14 KB | Display | |
Data in CIF | ![]() | 19.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11068.237 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.71 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 5% MPD, 4.25M Na Formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jul 18, 2008 Details: A fixed exit Si double crystal monochromator followed by a two dimensional focusing mirror which is coated in rhodium. | ||||||||||||
Radiation | Monochromator: Fixed exit Si double crystal monochromator / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.1→50 Å / Num. obs: 21026 / % possible obs: 99.6 % / Redundancy: 10.3 % / Biso Wilson estimate: 29.3 Å2 / Rmerge(I) obs: 0.067 / Net I/σ(I): 55.8 | ||||||||||||
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 8.5 % / Rmerge(I) obs: 0.309 / Mean I/σ(I) obs: 5.5 / Num. unique all: 1098 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 91.2801 Å2 / ksol: 0.4 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 47.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→22.68 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.017 / Total num. of bins used: 6
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Xplor file |
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