- PDB-3cmt: Mechanism of homologous recombination from the RecA-ssDNA/dsDNA s... -
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Basic information
Entry
Database: PDB / ID: 3cmt
Title
Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
Components
DNA (5'-D(*DTP*DTP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DCP*DTP*DTP*DTP*DT)-3')
DNA (5'-D(P*DGP*DGP*DTP*DGP*DGP*DG)-3')
Protein recA
Keywords
recombination/DNA / homologous recombination / ATP-binding / Cytoplasm / DNA damage / DNA recombination / DNA repair / DNA-binding / Nucleotide-binding / SOS response / Stress response / recombination-DNA COMPLEX
Function / homology
Function and homology information
DNA polymerase V complex / homologous recombination / SOS response / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / translesion synthesis / ATP-dependent activity, acting on DNA / cell motility / single-stranded DNA binding ...DNA polymerase V complex / homologous recombination / SOS response / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / translesion synthesis / ATP-dependent activity, acting on DNA / cell motility / single-stranded DNA binding / DNA-binding transcription factor binding / DNA recombination / damaged DNA binding / DNA damage response / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function
RecA protein, C-terminal domain / Rec A Protein; domain 2 / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / : / RecA C-terminal domain / recA signature. / DNA recombination and repair protein RecA / : / recA bacterial DNA recombination protein ...RecA protein, C-terminal domain / Rec A Protein; domain 2 / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / : / RecA C-terminal domain / recA signature. / DNA recombination and repair protein RecA / : / recA bacterial DNA recombination protein / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / P-loop containing nucleotide triphosphate hydrolases / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 2-Layer Sandwich / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology
B: DNA (5'-D(*DTP*DTP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DCP*DTP*DTP*DTP*DT)-3') C: DNA (5'-D(P*DGP*DGP*DTP*DGP*DGP*DG)-3') E: DNA (5'-D(*DTP*DTP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DCP*DTP*DTP*DTP*DT)-3') F: DNA (5'-D(P*DGP*DGP*DTP*DGP*DGP*DG)-3') A: Protein recA D: Protein recA hetero molecules
B: DNA (5'-D(*DTP*DTP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DCP*DTP*DTP*DTP*DT)-3') C: DNA (5'-D(P*DGP*DGP*DTP*DGP*DGP*DG)-3') A: Protein recA hetero molecules
E: DNA (5'-D(*DTP*DTP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DCP*DTP*DTP*DTP*DT)-3') F: DNA (5'-D(P*DGP*DGP*DTP*DGP*DGP*DG)-3') D: Protein recA hetero molecules
Density Matthews: 2.54 Å3/Da / Density % sol: 51.51 %
Crystal grow
pH: 8 Details: the RecA5 fusion protein was incubated with a 1.5-fold molar excess of the primary d(T5C3AC2T4) oligonucleotide in protein buffer supplemented with 2 mM ADP, 10 mM MgCl2 and 8 mM AlF4, pH 6. ...Details: the RecA5 fusion protein was incubated with a 1.5-fold molar excess of the primary d(T5C3AC2T4) oligonucleotide in protein buffer supplemented with 2 mM ADP, 10 mM MgCl2 and 8 mM AlF4, pH 6.0, for 30 min, followed by the addition of 2-fold molar excess of the complementary d(G2TG3) oligonucleotide. The crystals grew from 50 mM Tris-Cl, 9% (w/v) PVP K15, 32% (v/v) MPD, 100 mM magnesium acetate, 10 mM DTT, pH 8.0.
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