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Yorodumi- PDB-2zid: Crystal structure of dextran glucosidase E236Q complex with isoma... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2zid | |||||||||
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Title | Crystal structure of dextran glucosidase E236Q complex with isomaltotriose | |||||||||
Components | Dextran glucosidase | |||||||||
Keywords | HYDROLASE / TIM barrel / (beta/alpha)8-barrel | |||||||||
Function / homology | Function and homology information glucan 1,6-alpha-glucosidase / glucan 1,6-alpha-glucosidase activity / carbohydrate metabolic process / cytoplasm Similarity search - Function | |||||||||
Biological species | Streptococcus mutans (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | |||||||||
Authors | Hondoh, H. / Saburi, W. / Mori, H. / Okuyama, M. / Nakada, T. / Matsuura, Y. / Kimura, A. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 2008 Title: Substrate recognition mechanism of alpha-1,6-glucosidic linkage hydrolyzing enzyme, dextran glucosidase from Streptococcus mutans. Authors: Hondoh, H. / Saburi, W. / Mori, H. / Okuyama, M. / Nakada, T. / Matsuura, Y. / Kimura, A. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2zid.cif.gz | 130.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2zid.ent.gz | 98.1 KB | Display | PDB format |
PDBx/mmJSON format | 2zid.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zi/2zid ftp://data.pdbj.org/pub/pdb/validation_reports/zi/2zid | HTTPS FTP |
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-Related structure data
Related structure data | 2zicSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 63022.855 Da / Num. of mol.: 1 / Mutation: E236Q, N536L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus mutans (bacteria) / Gene: dexB / Plasmid: pET-23d / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) CodonPlus RIL References: UniProt: Q2HWU5, UniProt: Q99040*PLUS, glucan 1,6-alpha-glucosidase | ||||
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#2: Polysaccharide | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
#3: Chemical | #4: Water | ChemComp-HOH / | Sequence details | THE SEQUENCE OF THE RESIDUES 1-5 IS DESIGNED ACCORDING TO THE SEQUENCE OF THE RESIDUES 1-5 REPORTED ...THE SEQUENCE OF THE RESIDUES 1-5 IS DESIGNED ACCORDING TO THE SEQUENCE OF THE RESIDUES 1-5 REPORTED IN UNIPLOT Q99040, DEXB_STRMU (DEXTRAN GLUCOSIDAS | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.31 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 100mM tris-HCl, 12% PEG6000, 200mM NaCl, 25mM isomaltotriose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU ULTRAX 18 / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Feb 13, 2006 / Details: mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→36 Å / Num. obs: 32499 / % possible obs: 100 % / Biso Wilson estimate: 9.5 Å2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2ZIC Resolution: 2.2→10 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 2068439.45 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 73.2702 Å2 / ksol: 0.55 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 6
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Xplor file |
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