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Yorodumi- PDB-2yui: Solution structure of the N-terminal domain in human cytokine-ind... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2yui | ||||||
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Title | Solution structure of the N-terminal domain in human cytokine-induced apoptosis inhibitor anamorsin | ||||||
Components | Anamorsin | ||||||
Keywords | APOPTOSIS / Cytokine-induced apoptosis inhibitor 1 / CIAPIN1 / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information Cytosolic iron-sulfur cluster assembly / iron-sulfur cluster assembly / hemopoiesis / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / electron transfer activity / iron ion binding / nucleolus / negative regulation of apoptotic process ...Cytosolic iron-sulfur cluster assembly / iron-sulfur cluster assembly / hemopoiesis / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / electron transfer activity / iron ion binding / nucleolus / negative regulation of apoptotic process / apoptotic process / mitochondrion / nucleoplasm / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Saito, K. / Tomizawa, T. / Tochio, N. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the N-terminal domain in human cytokine-induced apoptosis inhibitor anamorsin Authors: Saito, K. / Tomizawa, T. / Tochio, N. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2yui.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2yui.ent.gz | 881 KB | Display | PDB format |
PDBx/mmJSON format | 2yui.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2yui_validation.pdf.gz | 341.7 KB | Display | wwPDB validaton report |
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Full document | 2yui_full_validation.pdf.gz | 499.2 KB | Display | |
Data in XML | 2yui_validation.xml.gz | 67.5 KB | Display | |
Data in CIF | 2yui_validation.cif.gz | 84 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yu/2yui ftp://data.pdbj.org/pub/pdb/validation_reports/yu/2yui | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 19199.701 Da / Num. of mol.: 1 / Fragment: N-terminal domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: cell-free protein synthesis / Gene: IOH4051 / Plasmid: P051025-15 / References: UniProt: Q6FI81 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1mM protein, 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 900 MHz |
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-Processing
NMR software |
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NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |