5S class rRNA transcription by RNA polymerase III / transcription factor TFIIIC complex / transcription initiation at RNA polymerase III promoter / phosphatase activity / transcription by RNA polymerase III / DNA binding / nucleus / cytoplasm Similarity search - Function
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.00849 Å / Relative weight: 1
Reflection
Resolution: 1.5→50 Å / Num. obs: 50268 / % possible obs: 99.5 % / Observed criterion σ(I): 0 / Redundancy: 4.05 % / Biso Wilson estimate: 15.411 Å2 / Rmerge(I) obs: 0.16 / Net I/σ(I): 15.5
Reflection shell
Resolution: 1.5→1.54 Å / Redundancy: 4.02 % / Rmerge(I) obs: 0.99 / Mean I/σ(I) obs: 2.19 / % possible all: 99.9
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Processing
Software
Name
Version
Classification
PHENIX
(PHENIX.REFINE)
refinement
XDS
datareduction
XSCALE
datascaling
SHARP
phasing
Refinement
Method to determine structure: SIRAS Starting model: NONE Resolution: 1.5→43.365 Å / SU ML: 0.16 / σ(F): 1.78 / Phase error: 17.15 / Stereochemistry target values: ML Details: RESIDUES 2-260 ARE VISIBLE IN THE ELECTRON DENSITY, EXCEPT FOR RESIDUES 216-225 WHICH ARE DISORDERED
Rfactor
Num. reflection
% reflection
Rfree
0.1826
2551
5.1 %
Rwork
0.1559
-
-
obs
0.1573
50251
99.44 %
Solvent computation
Shrinkage radii: 0.77 Å / VDW probe radii: 0.9 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 45.494 Å2 / ksol: 0.46 e/Å3