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- PDB-2y7l: Structure of N-terminal domain of Candida albicans Als9-2 in comp... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2y7l | ||||||
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Title | Structure of N-terminal domain of Candida albicans Als9-2 in complex with human fibrinogen gamma peptide | ||||||
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![]() | CELL ADHESION / ADHESIN / PEPTIDE BINDING PROTEIN | ||||||
Function / homology | ![]() cell adhesion involved in multi-species biofilm formation / platelet maturation / fungal-type cell wall / fibrinogen complex / Regulation of TLR by endogenous ligand / platelet alpha granule / blood coagulation, fibrin clot formation / cell adhesion involved in single-species biofilm formation / cellular response to interleukin-6 / MyD88 deficiency (TLR2/4) ...cell adhesion involved in multi-species biofilm formation / platelet maturation / fungal-type cell wall / fibrinogen complex / Regulation of TLR by endogenous ligand / platelet alpha granule / blood coagulation, fibrin clot formation / cell adhesion involved in single-species biofilm formation / cellular response to interleukin-6 / MyD88 deficiency (TLR2/4) / positive regulation of heterotypic cell-cell adhesion / IRAK4 deficiency (TLR2/4) / MyD88:MAL(TIRAP) cascade initiated on plasma membrane / extracellular matrix structural constituent / plasminogen activation / p130Cas linkage to MAPK signaling for integrins / positive regulation of peptide hormone secretion / positive regulation of exocytosis / GRB2:SOS provides linkage to MAPK signaling for Integrins / protein secretion / protein polymerization / cellular response to interleukin-1 / Integrin cell surface interactions / side of membrane / negative regulation of endothelial cell apoptotic process / Common Pathway of Fibrin Clot Formation / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / positive regulation of substrate adhesion-dependent cell spreading / positive regulation of vasoconstriction / cell adhesion molecule binding / fibrinolysis / Integrin signaling / cell-matrix adhesion / platelet alpha granule lumen / positive regulation of protein secretion / Post-translational protein phosphorylation / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / platelet activation / platelet aggregation / cell-cell adhesion / response to calcium ion / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Signaling by BRAF and RAF1 fusions / Platelet degranulation / ER-Phagosome pathway / protein-containing complex assembly / collagen-containing extracellular matrix / positive regulation of ERK1 and ERK2 cascade / blood microparticle / cell adhesion / endoplasmic reticulum lumen / external side of plasma membrane / signaling receptor binding / structural molecule activity / cell surface / extracellular space / extracellular exosome / extracellular region / identical protein binding / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Salgado, P.S. / Cota, E. | ||||||
![]() | ![]() Title: Structural Basis for the Broad Specificity to Host- Cell Ligands by the Pathogenic Fungus Candida Albicans. Authors: Salgado, P.S. / Yan, R. / Taylor, J.D. / Burchell, L. / Jones, R. / Hoyer, L.L. / Matthews, S.J. / Simpson, P.J. / Cota, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.4 KB | Display | ![]() |
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PDB format | ![]() | 59.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.9 KB | Display | ![]() |
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Full document | ![]() | 428.2 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 25.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2y7mSC ![]() 2y7nC ![]() 2y7oC ![]() 2ylhC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33599.988 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN, RESIDUES 18-328 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1691.780 Da / Num. of mol.: 1 / Fragment: RESIDUES 318-334 / Source method: obtained synthetically / Source: (synth.) ![]() |
#3: Water | ChemComp-HOH / |
Sequence details | SEQUENCE EXCLUDES N-TERMINAL SIGNAL SEQUENCE (18 RESIDUES) FULL 17MER PEPTIDE SEQUENCE ...SEQUENCE EXCLUDES N-TERMINAL SIGNAL SEQUENCE (18 RESIDUES) FULL 17MER PEPTIDE SEQUENCE GEGQQHHLGG |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45 % / Description: NONE |
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Crystal grow | pH: 8 / Details: 0.2MM AMMONIUM FLUORIDE, 20% PEG3350, PH 6.2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Nov 25, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 1.49→50 Å / Num. obs: 50160 / % possible obs: 98 % / Observed criterion σ(I): 9.3 / Redundancy: 13.7 % / Biso Wilson estimate: 11.8 Å2 / Rmerge(I) obs: 0.13 / Net I/σ(I): 36.4 |
Reflection shell | Resolution: 1.49→1.52 Å / Redundancy: 9.3 % / Rmerge(I) obs: 0.41 / Mean I/σ(I) obs: 9.3 / % possible all: 86.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2Y7M Resolution: 1.49→34.64 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.933 / SU B: 1.222 / SU ML: 0.048 / Cross valid method: THROUGHOUT / ESU R: 0.079 / ESU R Free: 0.082 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.735 Å2
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Refinement step | Cycle: LAST / Resolution: 1.49→34.64 Å
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