beta-amylase / beta-amylase activity / amylopectin maltohydrolase activity / polysaccharide catabolic process / identical protein binding 類似検索 - 分子機能
Glycoside hydrolase, family 14B, plant / Beta-amylase active site 2. / Glycoside hydrolase, family 14, conserved site / Beta-amylase active site 1. / Glycoside hydrolase, family 14 / Glycosyl hydrolase family 14 / Glycosidases / Glycoside hydrolase superfamily / TIM Barrel / Alpha-Beta Barrel / Alpha Beta 類似検索 - ドメイン・相同性
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
4-O-ALPHA-D-GLUCOPYRANOSYLMORANOLINE: THIS IS A DISACCHARIDE INHIBITOR COMPRISED OF ALPHA-1,4- ...4-O-ALPHA-D-GLUCOPYRANOSYLMORANOLINE: THIS IS A DISACCHARIDE INHIBITOR COMPRISED OF ALPHA-1,4-LINKED ALPHA-D-GLUCOSE AND 1-DEOXYNOJIRIMYCIN RESIDUES. IT WAS SOAKED INTO CRYSTALS AT 10 MM FOR 1 HOUR. 1,2-ETHANEDIOL: PRESENT AT 20 PERCENT IN THE CRYOPROTECTANT
配列の詳細
PROTEIN ISOLATED FROM NATURAL SOURCE BUT SEQUENCE DIFFERS FROM UNIPROTKB DATABASE ENTRY P16098 AT ...PROTEIN ISOLATED FROM NATURAL SOURCE BUT SEQUENCE DIFFERS FROM UNIPROTKB DATABASE ENTRY P16098 AT THREE POSITIONS. THESE CHANGES WERE IDENTIFIED FROM ELECTRON DENSITY MAPS AND ARE ATTRIBUTED TO NATURAL VARIATION
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 1.91 Å3/Da / 溶媒含有率: 35.6 % / 解説: NONE
結晶化
温度: 291 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.5 詳細: CRYSTALS WERE GROWN AT 291 K USING THE HANGING DROP VAPOUR DIFFUSION METHOD WITH PROTEIN AT 10 MG PER ML AND A PRECIPITANT COMPRISED OF 14 PERCENT PEG 3350 IN 100 MM BIS-TRIS PROPANE BUFFER AT PH 5.5
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RUH3R / 波長: 1.542
検出器
タイプ: MAR scanner 345 mm plate / 検出器: IMAGE PLATE / 日付: 2007年12月4日 / 詳細: MIRRORS
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.542 Å / 相対比: 1
反射
解像度: 1.79→21.81 Å / Num. obs: 41215 / % possible obs: 97.2 % / Observed criterion σ(I): -9 / 冗長度: 3.56 % / Biso Wilson estimate: 14.9 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 10.48
反射 シェル
解像度: 1.8→1.9 Å / 冗長度: 3.35 % / Rmerge(I) obs: 0.11 / Mean I/σ(I) obs: 6.31 / % possible all: 98.3
解像度: 1.8→21.115 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.95 / SU B: 4.46 / SU ML: 0.064 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.116 / ESU R Free: 0.111 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.1709
2070
5.07 %
RANDOM
Rwork
0.1277
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obs
0.13
41174
96.778 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK BULK SOLVENT