+Open data
-Basic information
Entry | Database: PDB / ID: 2w8x | ||||||
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Title | Structure of the tick ion-channel modulator Ra-KLP | ||||||
Components | ION-CHANNEL MODULATOR RAKLP | ||||||
Keywords | MEMBRANE PROTEIN / SALIVARY GLAND / KUNITZ DOMAINS / MAXIK CHANNEL ACTIVATION / SULPHUR SAD | ||||||
Function / homology | Pancreatic trypsin inhibitor Kunitz domain / Factor Xa Inhibitor / Few Secondary Structures / Irregular / ACETATE ION / IMIDAZOLE Function and homology information | ||||||
Biological species | RHIPICEPHALUS APPENDICULATUS (arthropod) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.6 Å | ||||||
Authors | Paesen, G.C. / Siebold, C. / Dallas, M. / Peers, C. / Harlos, K. / Nuttall, P.A. / Nunn, M.A. / Stuart, D.I. / Esnouf, R.M. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2009 Title: An Ion-Channel Modulator from the Saliva of the Brown Ear Tick Has a Highly Modified Kunitz/Bpti Structure. Authors: Paesen, G.C. / Siebold, C. / Dallas, M.L. / Peers, C. / Harlos, K. / Nuttall, P.A. / Nunn, M.A. / Stuart, D.I. / Esnouf, R.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2w8x.cif.gz | 72.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2w8x.ent.gz | 58.8 KB | Display | PDB format |
PDBx/mmJSON format | 2w8x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w8/2w8x ftp://data.pdbj.org/pub/pdb/validation_reports/w8/2w8x | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.2478, -0.17277, -0.95328), Vector: |
-Components
#1: Protein | Mass: 8419.331 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RHIPICEPHALUS APPENDICULATUS (arthropod) Plasmid: PET23A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): ORIGAMI(DE3) PLYSS #2: Chemical | ChemComp-GOL / | #3: Chemical | ChemComp-ACT / | #4: Chemical | ChemComp-IMD / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 35.91 % / Description: NONE |
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Crystal grow | pH: 7.5 / Details: 10% (V/V) ETHANOL, 1.5M NACL, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX14.1 / Wavelength: 1.488 |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→20 Å / Num. obs: 23817 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 40.2 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 62.8 |
Reflection shell | Resolution: 1.6→1.7 Å / Rmerge(I) obs: 0.29 / Mean I/σ(I) obs: 18.1 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD Starting model: NONE Resolution: 1.6→20 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.941 / SU B: 2.045 / SU ML: 0.034 / Cross valid method: THROUGHOUT / ESU R: 0.098 / ESU R Free: 0.074 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.67 Å2
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Refinement step | Cycle: LAST / Resolution: 1.6→20 Å
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Refine LS restraints |
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