- PDB-2w00: Crystal structure of the HsdR subunit of the EcoR124I restriction... -
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基本情報
登録情報
データベース: PDB / ID: 2w00
タイトル
Crystal structure of the HsdR subunit of the EcoR124I restriction enzyme in complex with ATP
要素
HSDR
キーワード
HYDROLASE / ATP-BINDING / DNA-BINDING / RESTRICTION SYSTEM / HELICASE / R.ECOR124I / NUCLEOTIDE-BINDING / TYPE I RESTRICTION-MODIFICATION ENZYME
機能・相同性
機能・相同性情報
type I site-specific deoxyribonuclease / type I site-specific deoxyribonuclease activity / DNA restriction-modification system / DNA binding / ATP binding 類似検索 - 分子機能
Arc Repressor Mutant, subunit A - #2110 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #2040 / tt1808, chain A - #50 / Actin; Chain A, domain 4 - #50 / tt1808, chain A / Restriction endonuclease, type I, HsdR, N-terminal / Type I restriction enzyme R protein, C-terminal / SWI2/SNF2 ATPase / Type I restriction enzyme R protein N terminus (HSDR_N) / Type I restriction and modification enzyme - subunit R C terminal ...Arc Repressor Mutant, subunit A - #2110 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #2040 / tt1808, chain A - #50 / Actin; Chain A, domain 4 - #50 / tt1808, chain A / Restriction endonuclease, type I, HsdR, N-terminal / Type I restriction enzyme R protein, C-terminal / SWI2/SNF2 ATPase / Type I restriction enzyme R protein N terminus (HSDR_N) / Type I restriction and modification enzyme - subunit R C terminal / SWI2/SNF2 ATPase / Restriction endonuclease, type I, HsdR / Actin; Chain A, domain 4 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase superfamily 1/2, ATP-binding domain / Arc Repressor Mutant, subunit A / P-loop containing nucleotide triphosphate hydrolases / Alpha-Beta Complex / Up-down Bundle / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta 類似検索 - ドメイン・相同性
ADENOSINE-5'-TRIPHOSPHATE / Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit / Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit 類似検索 - 構成要素
温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: 1 UL OF PROTEIN IN 20 MM PHOSPHATE PH 7.5, 100 MM KCL, 5 MM ATP WAS MIXED WITH 2 UL OF RESERVOIR, CONTAINING 0.2 M LI2SO4, 8 % PEG 20K, 8 % PEG 550 MME, 1.5 MM DTT, 277 K, SITTING DROP