- PDB-2w00: Crystal structure of the HsdR subunit of the EcoR124I restriction... -
+
Open data
ID or keywords:
Loading...
-
Basic information
Entry
Database: PDB / ID: 2w00
Title
Crystal structure of the HsdR subunit of the EcoR124I restriction enzyme in complex with ATP
Components
HSDR
Keywords
HYDROLASE / ATP-BINDING / DNA-BINDING / RESTRICTION SYSTEM / HELICASE / R.ECOR124I / NUCLEOTIDE-BINDING / TYPE I RESTRICTION-MODIFICATION ENZYME
Function / homology
Function and homology information
type I site-specific deoxyribonuclease / type I site-specific deoxyribonuclease activity / DNA restriction-modification system / DNA binding / ATP binding Similarity search - Function
Arc Repressor Mutant, subunit A - #2110 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #2040 / tt1808, chain A - #50 / Actin; Chain A, domain 4 - #50 / tt1808, chain A / Restriction endonuclease, type I, HsdR, N-terminal / Type I restriction enzyme R protein, C-terminal / SWI2/SNF2 ATPase / Type I restriction enzyme R protein N terminus (HSDR_N) / Type I restriction and modification enzyme - subunit R C terminal ...Arc Repressor Mutant, subunit A - #2110 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #2040 / tt1808, chain A - #50 / Actin; Chain A, domain 4 - #50 / tt1808, chain A / Restriction endonuclease, type I, HsdR, N-terminal / Type I restriction enzyme R protein, C-terminal / SWI2/SNF2 ATPase / Type I restriction enzyme R protein N terminus (HSDR_N) / Type I restriction and modification enzyme - subunit R C terminal / SWI2/SNF2 ATPase / Restriction endonuclease, type I, HsdR / Actin; Chain A, domain 4 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase superfamily 1/2, ATP-binding domain / Arc Repressor Mutant, subunit A / P-loop containing nucleotide triphosphate hydrolases / Alpha-Beta Complex / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta Similarity search - Domain/homology
ADENOSINE-5'-TRIPHOSPHATE / Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit / Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit Similarity search - Component
Biological species
ESCHERICHIA COLI (E. coli)
Method
X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.6 Å
Mass: 18.015 Da / Num. of mol.: 582 / Source method: isolated from a natural source / Formula: H2O
Sequence details
SEQUENCE TAKEN FROM THE NCBI PROTEIN DATABASE
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 3.1 Å3/Da / Density % sol: 60 % / Description: NONE
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 1 UL OF PROTEIN IN 20 MM PHOSPHATE PH 7.5, 100 MM KCL, 5 MM ATP WAS MIXED WITH 2 UL OF RESERVOIR, CONTAINING 0.2 M LI2SO4, 8 % PEG 20K, 8 % PEG 550 MME, 1.5 MM DTT, 277 K, SITTING DROP