- PDB-2vux: Human ribonucleotide reductase, subunit M2 B -
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IDまたはキーワード:
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基本情報
登録情報
データベース: PDB / ID: 2vux
タイトル
Human ribonucleotide reductase, subunit M2 B
要素
RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2 B
キーワード
OXIDOREDUCTASE / DNA REPLICATION / DE NOVO PATHWAY / NUCLEOTIDE METABOLISM / RIBONUCLEOTIDE REDUCTASE / RNR / IRON / P53R2 / CASP8 / NUCLEUS / METAL-BINDING / DNA DAMAGE / DNA REPAIR / IRON BINDING / SUBUNIT M2 B
機能・相同性
機能・相同性情報
deoxyribonucleoside triphosphate metabolic process / ribonucleoside diphosphate metabolic process / positive regulation of G0 to G1 transition / mitochondrial DNA replication / 2'-deoxyribonucleotide biosynthetic process / renal system process / negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator / ribonucleoside-diphosphate reductase complex / ribonucleoside-diphosphate reductase / ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor ...deoxyribonucleoside triphosphate metabolic process / ribonucleoside diphosphate metabolic process / positive regulation of G0 to G1 transition / mitochondrial DNA replication / 2'-deoxyribonucleotide biosynthetic process / renal system process / negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator / ribonucleoside-diphosphate reductase complex / ribonucleoside-diphosphate reductase / ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor / Interconversion of nucleotide di- and triphosphates / deoxyribonucleotide biosynthetic process / positive regulation of G2/M transition of mitotic cell cycle / DNA synthesis involved in DNA repair / response to amine / kidney development / TP53 Regulates Metabolic Genes / response to oxidative stress / DNA repair / mitochondrion / nucleoplasm / identical protein binding / metal ion binding / cytosol 類似検索 - 分子機能
Ribonucleotide reductase small subunit, acitve site / Ribonucleotide reductase small subunit signature. / Ribonucleotide reductase small subunit / Ribonucleotide reductase small subunit family / Ribonucleotide reductase, small chain / Ribonucleotide Reductase, subunit A / Ribonucleotide Reductase, subunit A / Ribonucleotide reductase-like / Ferritin-like superfamily / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
: / Ribonucleoside-diphosphate reductase subunit M2 B 類似検索 - 構成要素
PROTEIN WAS CO-CRYSTALLIZED IN THE PRESENCE OF CHYMOTRYPSIN.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.2 Å3/Da / 溶媒含有率: 60 % / 解説: NONE
結晶化
pH: 6.5 詳細: 0.1M NACACODYLATE PH 6.5, 2.3M AMMONIUM SULFATE, 0.2M SODIUM CHLORIDE. CHYMOTRYPSIN WAS ADDED TO A RATIO CHYMOTRYPSIN:PROTEIN OF 1:100 PRIOR TO CRYSTALLISATION
解像度: 2.8→19.83 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.911 / SU B: 30.141 / SU ML: 0.263 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / ESU R: 0.662 / ESU R Free: 0.338 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES 38-42, 100-105 IN SUBUNIT A AND 38REMARK
Rfactor
反射数
%反射
Selection details
Rfree
0.258
1831
8 %
RANDOM
Rwork
0.209
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obs
0.213
21049
100 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK