Monochromator: DIAMOND (111), GE(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.934 Å / Relative weight: 1
Reflection
Resolution: 2.2→20 Å / Num. obs: 10576 / % possible obs: 99.7 % / Observed criterion σ(I): 2 / Redundancy: 11.7 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 30.27
Reflection shell
Resolution: 2.2→2.3 Å / Redundancy: 8.9 % / Rmerge(I) obs: 0.52 / Mean I/σ(I) obs: 4.75 / % possible all: 100
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Processing
Software
Name
Version
Classification
REFMAC
5.3.0040
refinement
XDS
datareduction
XSCALE
datascaling
SOLVE
phasing
Refinement
Method to determine structure: MAD Starting model: NONE Resolution: 2.2→20 Å / Cor.coef. Fo:Fc: 0.947 / Cor.coef. Fo:Fc free: 0.902 / SU B: 10.977 / SU ML: 0.137 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.25 / ESU R Free: 0.211 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES 25-30 ARE DISORDERED, FIRST SER IS FROM VECTOR.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.25
503
4.8 %
RANDOM
Rwork
0.194
-
-
-
obs
0.196
10075
99.9 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK