+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2v9r | ||||||
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タイトル | First and second Ig domains from human Robo1 (Form 2) | ||||||
要素 | ROUNDABOUT HOMOLOG 1 | ||||||
キーワード | RECEPTOR / PROTO-ONCOGENE / DIFFERENTIATION / PHOSPHORYLATION / DISEASE MUTATION / NEURONAL DEVELOPMENT / IMMUNOGLOBULIN DOMAIN / CHEMOTAXIS / NEUROGENESIS / GLYCOPROTEIN / TRANSMEMBRANE / ROBO1 / MEMBRANE / IG DOMAIN / ROUNDABOUT / DEVELOPMENTAL PROTEIN | ||||||
機能・相同性 | 機能・相同性情報 chemorepulsion involved in postnatal olfactory bulb interneuron migration / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of mammary gland epithelial cell proliferation / LRR domain binding / negative regulation of chemokine-mediated signaling pathway / heart induction / axon guidance receptor activity / Netrin-1 signaling / positive regulation of vascular endothelial growth factor signaling pathway ...chemorepulsion involved in postnatal olfactory bulb interneuron migration / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of mammary gland epithelial cell proliferation / LRR domain binding / negative regulation of chemokine-mediated signaling pathway / heart induction / axon guidance receptor activity / Netrin-1 signaling / positive regulation of vascular endothelial growth factor signaling pathway / Role of ABL in ROBO-SLIT signaling / Regulation of commissural axon pathfinding by SLIT and ROBO / SLIT2:ROBO1 increases RHOA activity / Roundabout signaling pathway / Inactivation of CDC42 and RAC1 / endocardial cushion formation / Signaling by ROBO receptors / pulmonary valve morphogenesis / outflow tract septum morphogenesis / positive regulation of vascular endothelial growth factor receptor signaling pathway / cell migration involved in sprouting angiogenesis / aortic valve morphogenesis / Activation of RAC1 / axon midline choice point recognition / aorta development / positive regulation of Rho protein signal transduction / positive regulation of axonogenesis / ventricular septum morphogenesis / positive regulation of Notch signaling pathway / homophilic cell adhesion via plasma membrane adhesion molecules / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of MAP kinase activity / negative regulation of cell migration / activation of cysteine-type endopeptidase activity involved in apoptotic process / Regulation of expression of SLITs and ROBOs / nervous system development / cell adhesion / neuron projection / axon / negative regulation of gene expression / positive regulation of gene expression / cell surface / identical protein binding / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | HOMO SAPIENS (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 2 Å | ||||||
データ登録者 | Morlot, C. / Cusack, S. / McCarthy, A.A. | ||||||
引用 | ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 2007 タイトル: Structural Insights Into the Slit-Robo Complex. 著者: Morlot, C. / Thielens, N.M. / Ravelli, R.B. / Hemrika, W. / Romijn, R.A. / Gros, P. / Cusack, S. / Mccarthy, A.A. #1: ジャーナル: Acta Crystallogr.,Sect.F / 年: 2007 タイトル: Cloning, Expression, Crystallization and Preliminary X-Ray Analysis of the First Two Ig Domains from Human Roundabout 1 (Robo1). 著者: Morlot, C. / Hemrika, W. / Romijn, R.A. / Gros, P. / Cusack, S. / Mccarthy, A.A. | ||||||
履歴 |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2v9r.cif.gz | 55.4 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2v9r.ent.gz | 38.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2v9r.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/v9/2v9r ftp://data.pdbj.org/pub/pdb/validation_reports/v9/2v9r | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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Components on special symmetry positions |
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-要素
#1: タンパク質 | 分子量: 23587.717 Da / 分子数: 1 / 断片: IG1-2M, RESIDUES 61-266 / 変異: YES / 由来タイプ: 組換発現 / 由来: (組換発現) HOMO SAPIENS (ヒト) / 細胞株 (発現宿主): HEK293-EBNA1 / 発現宿主: HOMO SAPIENS (ヒト) / 参照: UniProt: Q9Y6N7 | ||||||
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#2: 化合物 | #3: 水 | ChemComp-HOH / | 構成要素の詳細 | ENGINEERED | 配列の詳細 | A N160D MUTATION WAS MADE TO PREVENT POTENTIAL GLYCOSYLAT | |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.1 Å3/Da / 溶媒含有率: 42 % / 解説: NONE |
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結晶化 | pH: 5.6 / 詳細: 12% PEG 6000, 0.1M LISO4, 0.1M NA CITRATE, pH 5.6 |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: ESRF / ビームライン: ID14-4 / 波長: 0.939 |
検出器 | タイプ: ADSC CCD / 検出器: CCD / 日付: 2006年11月3日 / 詳細: MIRROR |
放射 | モノクロメーター: SI(111) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.939 Å / 相対比: 1 |
反射 | 解像度: 2→30 Å / Num. obs: 54927 / % possible obs: 97.7 % / Observed criterion σ(I): 0 / 冗長度: 3.8 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 15.6 |
反射 シェル | 解像度: 2→2.1 Å / 冗長度: 3.9 % / Rmerge(I) obs: 0.2 / Mean I/σ(I) obs: 7 / % possible all: 94.1 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: PDB ENTRY 2V9Q 解像度: 2→30 Å / Cor.coef. Fo:Fc: 0.894 / Cor.coef. Fo:Fc free: 0.837 / SU B: 15.071 / SU ML: 0.218 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / ESU R: 0.243 / ESU R Free: 0.232 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THIS IS A SECOND CRYSTAL FORM OF THE SUBMISSION ABOVE, ROBO1 IG1-2. ALTHOUGH IT DIFFRACTED TO A HIGHER RESOLUTION THE R AND RFREE ARE LARGER ...詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THIS IS A SECOND CRYSTAL FORM OF THE SUBMISSION ABOVE, ROBO1 IG1-2. ALTHOUGH IT DIFFRACTED TO A HIGHER RESOLUTION THE R AND RFREE ARE LARGER THAN EXPECTED FOR THIS RESOLUTION. THIS IS PROBABLY DUE TO THE PRESENCE OF A WEAK SUPER LATTICE THAT WAS OBSERVED IN THE DIFFRACTION PATTERN.
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 18.74 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2→30 Å
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拘束条件 |
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