guanine deaminase / guanine deaminase activity / guanine catabolic process / deoxyguanosine catabolic process / dGMP catabolic process / GMP catabolic process / amide catabolic process / guanine metabolic process / Purine catabolism / allantoin metabolic process ...guanine deaminase / guanine deaminase activity / guanine catabolic process / deoxyguanosine catabolic process / dGMP catabolic process / GMP catabolic process / amide catabolic process / guanine metabolic process / Purine catabolism / allantoin metabolic process / nucleobase-containing compound metabolic process / nervous system development / zinc ion binding / cytosol Similarity search - Function
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
Mass: 53620.555 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: THE PROTEIN CHAIN CONTAINS A HEXAHISTIDINE TAIL IN THE N-TERMINUS Source: (gene. exp.) HOMO SAPIENS (human) / Description: MAMMALIAN GENE COLLECTION (MGC) / Plasmid: PNIC-BSA4 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9Y2T3, guanine deaminase
Mass: 18.015 Da / Num. of mol.: 193 / Source method: isolated from a natural source / Formula: H2O
Sequence details
OUR CLONED CONSTRUCT CONTAINS A HEXAHISTIDINE TAG AND A LINKER SEQUENCE IN THE N-TERMINUS ...OUR CLONED CONSTRUCT CONTAINS A HEXAHISTIDINE TAG AND A LINKER SEQUENCE IN THE N-TERMINUS (MHHHHHHSSGVDLGTENLYFQS).
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.65 Å3/Da / Density % sol: 53.14 % / Description: NONE
Resolution: 2.3→30 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.934 / SU B: 6.542 / SU ML: 0.158 / Cross valid method: THROUGHOUT / ESU R: 0.269 / ESU R Free: 0.209 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.222
1241
5.1 %
RANDOM
Rwork
0.169
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obs
0.172
23120
99.9 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK