Pseudoazurin with engineered amicyanin ligand loop, reduced form, pH 5.5
要素
PSEUDOAZURIN
キーワード
ELECTRON TRANSPORT / TYPE-1 COPPER / METAL-BINDING / REDOX POTENTIAL / COPPER / TRANSPORT / CUPREDOXIN / PERIPLASMIC / SPECTROSCOPIC PROPERTIES / LOOP SHORTENING / PROTEIN SCAFFOLD / ELECTRON TRANSFER
機能・相同性
機能・相同性情報
electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity / periplasmic space / copper ion binding 類似検索 - 分子機能
Pseudoazurin / Amicyanin/Pseudoazurin / Blue (type 1) copper protein, plastocyanin-type / Blue (type 1) copper domain / Copper binding proteins, plastocyanin/azurin family / Blue (type 1) copper protein, binding site / Type-1 copper (blue) proteins signature. / Cupredoxins - blue copper proteins / Cupredoxin / Immunoglobulin-like ...Pseudoazurin / Amicyanin/Pseudoazurin / Blue (type 1) copper protein, plastocyanin-type / Blue (type 1) copper domain / Copper binding proteins, plastocyanin/azurin family / Blue (type 1) copper protein, binding site / Type-1 copper (blue) proteins signature. / Cupredoxins - blue copper proteins / Cupredoxin / Immunoglobulin-like / Sandwich / Mainly Beta 類似検索 - ドメイン・相同性
THIS SEQUENCE CONTAINS THE SIGNAL PEPTIDE SEQUENCE. THE MATURE SEQUENCE STARTS AT A29 AND HAS 124 ...THIS SEQUENCE CONTAINS THE SIGNAL PEPTIDE SEQUENCE. THE MATURE SEQUENCE STARTS AT A29 AND HAS 124 AMINO ACID REDIDUES.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.09 Å3/Da / 溶媒含有率: 40.8 % / 解説: NONE
結晶化
手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.5 詳細: HANGING DROP VAPOR DIFFUSION METHOD, 1 MICRO-LITER OF PROTEIN (20 MG/ML IN 100 MM MES, PH 5.5 PLUS 30 MM NACL) WAS MIXED WITH 1 MICO-L OF RESERVOIR SOLUTION (100 MM MES, PH 5.5 PLUS 2 M ...詳細: HANGING DROP VAPOR DIFFUSION METHOD, 1 MICRO-LITER OF PROTEIN (20 MG/ML IN 100 MM MES, PH 5.5 PLUS 30 MM NACL) WAS MIXED WITH 1 MICO-L OF RESERVOIR SOLUTION (100 MM MES, PH 5.5 PLUS 2 M AMMONIUM SULFATE AND 2 M NACL). THE CRYSTALS WERE REDUCED BY ADDING 50 MM MERCAPTOETHANOL TO THE RESERVOIR SOLUTION AND BECAME COLORLESS AFTER 3 HOURS.
解像度: 1.99→30 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.931 / SU B: 6.968 / SU ML: 0.107 / 交差検証法: THROUGHOUT / ESU R: 0.175 / ESU R Free: 0.171 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. SOME SIDE CHAINS WERE MODELED WITH TWO ALTERNATE CONFORMATIONS. THE COPPER AND SULFUR ATOMS WERE REFINED ANISOTROPICALLY.
Rfactor
反射数
%反射
Selection details
Rfree
0.23
384
4.7 %
RANDOM
Rwork
0.16
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obs
0.163
7756
97 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK