Pseudoazurin with engineered amicyanin ligand loop, oxidized form, pH 7.5
要素
PSEUDOAZURIN
キーワード
ELECTRON TRANSPORT / TYPE-1 COPPER / METAL-BINDING / REDOX POTENTIAL / COPPER / TRANSPORT / CUPREDOXIN / PERIPLASMIC / SPECTROSCOPIC PROPERTIES / LOOP SHORTENING / PROTEIN SCAFFOLD / ELECTRON TRANSFER
機能・相同性
機能・相同性情報
electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity / periplasmic space / copper ion binding 類似検索 - 分子機能
Pseudoazurin / Amicyanin/Pseudoazurin / Blue (type 1) copper protein, plastocyanin-type / Blue (type 1) copper domain / Copper binding proteins, plastocyanin/azurin family / Blue (type 1) copper protein, binding site / Type-1 copper (blue) proteins signature. / Cupredoxins - blue copper proteins / Cupredoxin / Immunoglobulin-like ...Pseudoazurin / Amicyanin/Pseudoazurin / Blue (type 1) copper protein, plastocyanin-type / Blue (type 1) copper domain / Copper binding proteins, plastocyanin/azurin family / Blue (type 1) copper protein, binding site / Type-1 copper (blue) proteins signature. / Cupredoxins - blue copper proteins / Cupredoxin / Immunoglobulin-like / Sandwich / Mainly Beta 類似検索 - ドメイン・相同性
THIS SEQUENCE CONTAINS THE SIGNAL PEPTIDE SEQUENCE. THE MATURE SEQUENCE STARTS AT A29 AND HAS 124 ...THIS SEQUENCE CONTAINS THE SIGNAL PEPTIDE SEQUENCE. THE MATURE SEQUENCE STARTS AT A29 AND HAS 124 AMINO ACID REDIDUES.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.04 Å3/Da / 溶媒含有率: 39.16 % / 解説: NONE
結晶化
手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.5 詳細: HANGING DROP VAPOR DIFFUSION METHOD, MIXING 1MICRO-L OF PROTEIN (20 MG/ML IN 50 MM TRIS/HCL, PH 7.5 PLUS 30 MM NACL) WITH 1 MICRO-L RESERVOIR SOLUTION (10 MM TRIS/HCL, PH 7.5 PLUS 2 M ...詳細: HANGING DROP VAPOR DIFFUSION METHOD, MIXING 1MICRO-L OF PROTEIN (20 MG/ML IN 50 MM TRIS/HCL, PH 7.5 PLUS 30 MM NACL) WITH 1 MICRO-L RESERVOIR SOLUTION (10 MM TRIS/HCL, PH 7.5 PLUS 2 M AMMONIUM SULFATE PLUS 2 M NACL).
解像度: 1.3→20 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.955 / SU B: 1.995 / SU ML: 0.043 / 交差検証法: THROUGHOUT / ESU R: 0.057 / ESU R Free: 0.061 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. LOOP A10 TO A15 HAS BEEN MODELED WITH TWO MAIN CHAIN CONFORMATIONS. SOME SIDE CHAINS HAVE BEEN MODELED WITH TWO ALTERNATE SIDE CHAIN ...詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. LOOP A10 TO A15 HAS BEEN MODELED WITH TWO MAIN CHAIN CONFORMATIONS. SOME SIDE CHAINS HAVE BEEN MODELED WITH TWO ALTERNATE SIDE CHAIN CONFORMATIONS. THE COPPER AND SULFUR ATOMS HAVE BEEN REFINED ANISOTROPICALLY.
Rfactor
反射数
%反射
Selection details
Rfree
0.226
1467
5.1 %
RANDOM
Rwork
0.193
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obs
0.195
27344
94.5 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK