CollagentypeIValpha-3-bindingprotein / Ceramide transfer protein / hCERT / Goodpasture antigen-binding protein / GPBP / START domain- ...Ceramide transfer protein / hCERT / Goodpasture antigen-binding protein / GPBP / START domain-containing protein 11 / StARD11 / StAR-related lipid transfer protein 11
Mass: 11180.384 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 24-117 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: COL4A3BP, CERT, STARD11 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9Y5P4
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
2
3D HN(CA)CB
1
3
2
3DCBCA(CO)NH
1
4
2
3D HNCO
1
5
2
3DHN(CO)CA
1
6
1
3D 1H-15N NOESY
1
7
2
4D 13C 15N NOESY
1
8
2
3D (H)CCH-TOCSY
1
9
2
2D 1H-13C HSQC
1
10
2
3D 1H-13C NOESY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.2 mM [U-98% 15N] CERT-1, 10 mM HEPES-2, 100 mM sodium chloride-3, 5 mM DTT-4, 93% H2O/7% D2O
93% H2O/7% D2O
2
0.2 mM [U-98% 13C; U-98% 15N] CERT-5, 10 mM HEPES-6, 100 mM sodium chloride-7, 5 mM DTT-8, 93% H2O/7% D2O
93% H2O/7% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.2mM
CERT-1
[U-98% 15N]
1
10mM
HEPES-2
1
100mM
sodium chloride-3
1
5mM
DTT-4
1
0.2mM
CERT-5
[U-98% 13C; U-98% 15N]
2
10mM
HEPES-6
2
100mM
sodium chloride-7
2
5mM
DTT-8
2
Sample conditions
pH: 7.20 / Pressure: ambient / Temperature: 298 K
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NMR measurement
NMR spectrometer
Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz
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Processing
NMR software
Name
Developer
Classification
CYANA
Guntert, MumenthalerandWuthrich
structuresolution
CYANA
refinement
Refinement
Method: distance geometry / Software ordinal: 1
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1
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