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- PDB-2rk8: The Structure of rat cytosolic PEPCK in complex with phosphonoformate -

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Basic information

Entry
Database: PDB / ID: 2rk8
TitleThe Structure of rat cytosolic PEPCK in complex with phosphonoformate
ComponentsPhosphoenolpyruvate carboxykinase, cytosolic [GTP]
KeywordsLYASE / kinase / gluconeogenesis / Decarboxylase / GTP-binding / Nucleotide-binding
Function / homology
Function and homology information


Gluconeogenesis / phosphoenolpyruvate carboxykinase activity / cellular response to potassium ion starvation / protein serine kinase activity (using GTP as donor) / response to methionine / Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases / glycerol biosynthetic process from pyruvate / phosphoenolpyruvate carboxykinase (GTP) / phosphoenolpyruvate carboxykinase (GTP) activity / propionate catabolic process ...Gluconeogenesis / phosphoenolpyruvate carboxykinase activity / cellular response to potassium ion starvation / protein serine kinase activity (using GTP as donor) / response to methionine / Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases / glycerol biosynthetic process from pyruvate / phosphoenolpyruvate carboxykinase (GTP) / phosphoenolpyruvate carboxykinase (GTP) activity / propionate catabolic process / cellular response to raffinose / tricarboxylic acid metabolic process / regulation of lipid biosynthetic process / response to interleukin-6 / cellular response to fructose stimulus / cellular hypotonic response / carboxylic acid binding / cellular hypotonic salinity response / cellular response to phorbol 13-acetate 12-myristate / oxaloacetate metabolic process / hepatocyte differentiation / positive regulation of memory T cell differentiation / cellular hyperosmotic response / glyceraldehyde-3-phosphate biosynthetic process / nucleoside diphosphate kinase activity / cellular hyperosmotic salinity response / response to lipid / cellular response to glucagon stimulus / response to starvation / cellular response to interleukin-1 / positive regulation of lipid biosynthetic process / cellular response to retinoic acid / cellular response to cAMP / response to nutrient levels / cellular response to dexamethasone stimulus / response to activity / gluconeogenesis / cellular response to glucose stimulus / response to bacterium / response to insulin / lipid metabolic process / cellular response to insulin stimulus / glucose metabolic process / GDP binding / glucose homeostasis / cellular response to tumor necrosis factor / manganese ion binding / cellular response to hypoxia / peptidyl-serine phosphorylation / response to lipopolysaccharide / GTP binding / magnesium ion binding / endoplasmic reticulum / positive regulation of transcription by RNA polymerase II / cytoplasm / cytosol
Similarity search - Function
Phosphoenolpyruvate carboxykinase, GTP-utilising / Phosphoenolpyruvate carboxykinase, GTP-utilising, conserved site / Phosphoenolpyruvate carboxykinase, C-terminal P-loop domain / Phosphoenolpyruvate carboxykinase, GTP-utilising, N-terminal / Phosphoenolpyruvate carboxykinase C-terminal P-loop domain / Phosphoenolpyruvate carboxykinase N-terminal domain / Phosphoenolpyruvate carboxykinase (GTP) signature. / Phosphoenolpyruvate Carboxykinase; domain 2 / Phosphoenolpyruvate Carboxykinase, domain 2 / Phosphoenolpyruvate Carboxykinase; domain 1 ...Phosphoenolpyruvate carboxykinase, GTP-utilising / Phosphoenolpyruvate carboxykinase, GTP-utilising, conserved site / Phosphoenolpyruvate carboxykinase, C-terminal P-loop domain / Phosphoenolpyruvate carboxykinase, GTP-utilising, N-terminal / Phosphoenolpyruvate carboxykinase C-terminal P-loop domain / Phosphoenolpyruvate carboxykinase N-terminal domain / Phosphoenolpyruvate carboxykinase (GTP) signature. / Phosphoenolpyruvate Carboxykinase; domain 2 / Phosphoenolpyruvate Carboxykinase, domain 2 / Phosphoenolpyruvate Carboxykinase; domain 1 / Phosphoenolpyruvate Carboxykinase, domain 1 / Phosphoenolpyruvate Carboxykinase; domain 3 - #20 / Phosphoenolpyruvate carboxykinase, C-terminal / Phosphoenolpyruvate carboxykinase, N-terminal / Phosphoenolpyruvate Carboxykinase; domain 3 / Beta Complex / Alpha-Beta Complex / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
FORMIC ACID / : / DI(HYDROXYETHYL)ETHER / PHOSPHONOFORMIC ACID / Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
MethodX-RAY DIFFRACTION / Resolution: 2 Å
AuthorsSullivan, S.M. / Stiffin, R.M. / Carlson, G.M. / Holyoak, T.
CitationJournal: Biochemistry / Year: 2008
Title: Differential Inhibition of Cytosolic PEPCK by Substrate Analogues. Kinetic and Structural Characterization of Inhibitor Recognition.
Authors: Stiffin, R.M. / Sullivan, S.M. / Carlson, G.M. / Holyoak, T.
History
DepositionOct 16, 2007Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 29, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.2Oct 25, 2017Group: Refinement description / Category: software
Revision 1.3Feb 21, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
B: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
hetero molecules


Theoretical massNumber of molelcules
Total (without water)139,89411
Polymers139,2882
Non-polymers6069
Water15,115839
1
A: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
hetero molecules


Theoretical massNumber of molelcules
Total (without water)70,0006
Polymers69,6441
Non-polymers3565
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
hetero molecules


Theoretical massNumber of molelcules
Total (without water)69,8945
Polymers69,6441
Non-polymers2504
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)62.251, 119.470, 86.870
Angle α, β, γ (deg.)90.00, 107.06, 90.00
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Phosphoenolpyruvate carboxykinase, cytosolic [GTP] / Phosphoenolpyruvate carboxylase / PEPCK-C


Mass: 69643.789 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Pck1 / Plasmid: PGEX4T2 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3)
References: UniProt: P07379, phosphoenolpyruvate carboxykinase (GTP)

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Non-polymers , 6 types, 848 molecules

#2: Chemical ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mn
#3: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Na
#4: Chemical ChemComp-PPF / PHOSPHONOFORMIC ACID


Mass: 126.005 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: CH3O5P / Comment: medication, antivirus, inhibitor*YM
#5: Chemical ChemComp-FMT / FORMIC ACID


Mass: 46.025 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: CH2O2
#6: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 839 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.22 Å3/Da / Density % sol: 44.52 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.4
Details: 12-30% PEG 3350, 0.1M HEPES,10 MM MNCL2, pH 7.4, temperature 298K, VAPOR DIFFUSION, HANGING DROP

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418
DetectorType: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Jun 16, 2007 / Details: BLUE OSMIC Confocal MIRRORS
RadiationMonochromator: Blue osmic mirrors / Protocol: SINGLE WAVELENGTH / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 2→100 Å / Num. obs: 80055 / % possible obs: 98.2 % / Redundancy: 5.7 % / Rmerge(I) obs: 0.715 / Rsym value: 0.516
Reflection shellResolution: 2→2.07 Å / % possible all: 92.8

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
REFMAC5.2.0019refinement
PDB_EXTRACT3data extraction
CrystalCleardata collection
RefinementResolution: 2→30.06 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.928 / SU B: 9.712 / SU ML: 0.143 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.231 / ESU R Free: 0.193 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.249 4000 5 %RANDOM
Rwork0.195 ---
obs0.198 80020 98.18 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parametersBiso mean: 19.162 Å2
Baniso -1Baniso -2Baniso -3
1--0.04 Å20 Å20.02 Å2
2--0.03 Å20 Å2
3---0.02 Å2
Refinement stepCycle: LAST / Resolution: 2→30.06 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9691 0 31 839 10561
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0080.02210290
X-RAY DIFFRACTIONr_angle_refined_deg1.1171.96413991
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.64251322
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.93724.313473
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.79151810
X-RAY DIFFRACTIONr_dihedral_angle_4_deg15.2061563
X-RAY DIFFRACTIONr_chiral_restr0.0760.21465
X-RAY DIFFRACTIONr_gen_planes_refined0.0030.027944
X-RAY DIFFRACTIONr_nbd_refined0.1840.25032
X-RAY DIFFRACTIONr_nbtor_refined0.3020.26911
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1220.2886
X-RAY DIFFRACTIONr_metal_ion_refined0.1570.26
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.1710.2130
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.1640.247
X-RAY DIFFRACTIONr_mcbond_it0.3851.56282
X-RAY DIFFRACTIONr_mcangle_it0.704210164
X-RAY DIFFRACTIONr_scbond_it0.99234197
X-RAY DIFFRACTIONr_scangle_it1.6364.53781
LS refinement shellResolution: 2→2.054 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.345 261 -
Rwork0.272 5179 -
all-5440 -
obs--91.08 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
111.141.6667-6.49071.5031.916810.434-0.1261-0.28740.4935-0.17720.32880.27320.9087-0.0921-0.20270.09220.00970.0679-0.0144-0.07720.064155.987817.812-17.4886
21.02480.7519-0.4551.99220.03612.1974-0.01510.0128-0.0339-0.00280.0849-0.199-0.09350.1919-0.0698-0.01690.0066-0.01290.0533-0.01540.073646.628217.4969-4.3953
30.394-0.1976-0.44061.78070.49341.3270.07730.04070.0547-0.0529-0.11360.0921-0.2373-0.10330.03640.0301-0.00460.00180.0267-0.01470.057725.362918.11212.2248
43.56421.75750.19343.7939-0.13141.6336-0.0599-0.03750.0699-0.29310.1733-0.02470.0037-0.0027-0.11340.0737-0.0008-0.00750.0621-0.0091-0.006926.1222-10.3094-13.9012
50.65850.4746-0.42061.84450.23411.8161-0.03180.09790.0568-0.1015-0.0317-0.09420.00240.14020.0635-0.00590.0055-0.02140.05290.00830.037237.118910.1152-9.5295
60.35110.09710.05421.17570.27160.60080.05120.02330.03590.1292-0.0374-0.01490.0680.0483-0.01380.0348-0.0099-0.0280.070.00660.054539.080510.25130.1474
72.0651-1.68290.16133.85480.92181.70770.15470.21-0.1208-0.0274-0.24830.3053-0.06770.00210.0937-0.0034-0.01840.01110.05540.00630.019328.8410.1809-9.3535
80.2870.185-0.22660.4599-0.37410.33160.03640.01570.01020.0241-0.00660.0641-0.03230.0447-0.02970.0599-0.0222-0.00780.0843-0.01340.046728.612-3.22618.3774
90.21660.03970.20750.477-0.51950.86050.069-0.09510.06070.0534-0.0185-0.0118-0.13420.0976-0.05050.0706-0.01250.00370.0643-0.00880.04127.6326-2.705317.5226
101.444-1.39040.61643.48740.12230.5016-0.03090.0140.02980.5830.0120.4156-0.21990.06960.01890.1139-0.00820.1376-0.025-0.03180.104915.485317.622815.4728
110.4677-0.03190.66761.5201-0.69371.22960.0614-0.07460.08930.3182-0.09540.33-0.1841-0.03940.03390.0458-0.00660.06230.0631-0.03540.096519.853211.49911.3112
120.57950.08160.0590.3215-0.23330.3834-0.0269-0.0054-0.0099-0.03810.0454-0.0171-0.0414-0.046-0.01860.05550.0056-0.01070.0533-0.0140.062519.3974-12.08764.6288
130.75760.0645-0.25740.08630.21812.29560.0194-0.0844-0.05560.0428-0.0353-0.0197-0.1187-0.09810.0160.02250.0084-0.0120.04160.00480.050116.3649-12.253517.0183
141.19970.24791.66114.78242.24893.16320.2062-0.2344-0.4310.3944-0.23360.34910.0035-0.43630.02740.0178-0.03790.0590.08360.07870.03211.1682-18.903224.2017
151.13390.1896-0.57051.0712-0.07251.3207-0.04310.0511-0.1697-0.23380.03370.12360.0392-0.21690.0093-0.00250.0022-0.06190.0525-0.00230.079311.3724-22.44392.7631
1623.39636.1479-34.363.0273-9.572350.67050.4525-0.51991.5134-0.77090.6930.20271.9518-0.4399-1.14540.1791-0.1430.09790.1762-0.06660.018636.973119.1542-57.7463
170.9139-0.28050.22653.47210.20562.78370.02840.0909-0.0683-0.22860.1179-0.4666-0.05050.3052-0.1463-0.0277-0.03030.03430.0649-0.01250.107530.496215.1352-48.309
181.03190.0628-0.50790.93460.30721.04950.1180.04830.15120.1346-0.0690.135-0.15240.0546-0.0490.0490.0049-0.00850.0098-0.00630.067711.069221.8639-37.3118
190.75781.11230.40353.4689-0.22030.5744-0.12740.0325-0.0293-0.31210.0853-0.01540.07280.00950.0420.0558-0.0196-0.00530.09-0.01540.026910.1572-6.1213-56.8362
200.170.1569-0.12531.01670.11360.67390.0254-0.03310.03450.0346-0.0603-0.04560.00650.01770.03480.0367-0.0243-0.02610.07810.00940.051419.386711.2754-40.7072
210.5940.4173-0.1391.45650.86410.82750.05060.1212-0.0603-0.1206-0.07270.0789-0.0326-0.00450.0221-0.0006-0.0094-0.02040.05930.00840.024315.245411.9508-49.1122
220.11550.01280.1592.3929-0.52880.34380.0652-0.0438-0.0013-0.0267-0.108-0.00360.02040.05320.04280.0362-0.014-0.02270.1063-0.00410.030314.5092-1.0515-43.0048
232.49720.47340.03510.52920.48041.47540.1767-0.150.13170.0551-0.11690.0799-0.14440.0787-0.05980.1359-0.0390.03650.0079-0.01140.00962.3913-4.5399-20.1495
240.30170.01970.06591.3719-0.34790.12280.065-0.06330.06280.2272-0.0780.14620.00370.04970.0130.0712-0.04180.03550.0593-0.03970.0395.16036.521-28.0877
252.38210.8805-1.13764.580.07736.90790.11270.02630.12130.27160.05050.7909-0.3415-0.3941-0.1633-0.12390.03840.0785-0.0472-0.06790.2458-9.906921.4011-31.1687
261.2185-0.83971.45592.4468-1.53672.46760.0652-0.10040.03290.25920.04140.4654-0.1373-0.21-0.10660.0106-0.00050.08880.0699-0.0560.1573-3.285114.0945-32.0343
270.39110.3663-0.0090.34340.00810.70040.03420.0272-0.02170.0459-0.01480.0333-0.08170.0127-0.01950.07050.016-0.00190.0516-0.00580.04792.558-12.5132-35.2092
281.60250.1154-0.23280.33180.20711.97880.17670.05060.01710.1504-0.15520.0774-0.1454-0.0442-0.02150.06980.00570.01650.0275-0.01210.0338-3.9654-12.2315-24.801
290.31060.26750.74616.72470.13871.84890.2327-0.1729-0.260.6159-0.11390.57410.1341-0.1548-0.11880.0448-0.06020.09060.07930.00660.0248-9.0497-18.9824-18.0606
300.63810.3389-0.2890.735-0.3440.9778-0.02540.0826-0.1366-0.07010.02320.13110.018-0.10990.00210.02770.0135-0.02870.0434-0.01360.0828-7.4479-22.8348-39.396
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1AA1 - 93 - 11
2X-RAY DIFFRACTION2AA10 - 5412 - 56
3X-RAY DIFFRACTION3AA55 - 9657 - 98
4X-RAY DIFFRACTION4AA97 - 11999 - 121
5X-RAY DIFFRACTION5AA120 - 149122 - 151
6X-RAY DIFFRACTION6AA150 - 204152 - 206
7X-RAY DIFFRACTION7AA205 - 227207 - 229
8X-RAY DIFFRACTION8AA228 - 296230 - 298
9X-RAY DIFFRACTION9AA297 - 341299 - 343
10X-RAY DIFFRACTION10AA342 - 385344 - 387
11X-RAY DIFFRACTION11AA386 - 426388 - 428
12X-RAY DIFFRACTION12AA427 - 499429 - 501
13X-RAY DIFFRACTION13AA500 - 543502 - 545
14X-RAY DIFFRACTION14AA544 - 571546 - 573
15X-RAY DIFFRACTION15AA572 - 622574 - 624
16X-RAY DIFFRACTION16BB4 - 116 - 13
17X-RAY DIFFRACTION17BB12 - 4014 - 42
18X-RAY DIFFRACTION18BB41 - 8943 - 91
19X-RAY DIFFRACTION19BB90 - 12792 - 129
20X-RAY DIFFRACTION20BB128 - 179130 - 181
21X-RAY DIFFRACTION21BB180 - 224182 - 226
22X-RAY DIFFRACTION22BB225 - 264227 - 266
23X-RAY DIFFRACTION23BB265 - 307267 - 309
24X-RAY DIFFRACTION24BB308 - 370310 - 372
25X-RAY DIFFRACTION25BB371 - 388373 - 390
26X-RAY DIFFRACTION26BB389 - 418391 - 420
27X-RAY DIFFRACTION27BB419 - 499421 - 501
28X-RAY DIFFRACTION28BB500 - 544502 - 546
29X-RAY DIFFRACTION29BB545 - 571547 - 573
30X-RAY DIFFRACTION30BB572 - 622574 - 624

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