|Entry||Database: PDB / ID: 2rgc|
|Title||Crystal structure of H-RasQ61V-GppNHp|
|Keywords||ONCOPROTEIN / MOLECULAR SWITCH PROTEIN / ONCOGENE / SIGNALING PROTEIN / GTPASE / Disease mutation / Golgi apparatus / GTP-binding / Lipoprotein / Membrane / Methylation / Nucleotide-binding / Palmitate / Prenylation / Proto-oncogene|
|Function / homology|
Function and homology information
GTPase complex / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of ruffle assembly / regulation of MAP kinase activity / positive regulation of miRNA metabolic process / T-helper 1 type immune response / positive regulation of wound healing / negative regulation of GTPase activity / defense response to protozoan / Signaling by RAS GAP mutants ...GTPase complex / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of ruffle assembly / regulation of MAP kinase activity / positive regulation of miRNA metabolic process / T-helper 1 type immune response / positive regulation of wound healing / negative regulation of GTPase activity / defense response to protozoan / Signaling by RAS GAP mutants / Signaling by RAS GTPase mutants / Activation of RAS in B cells / RAS signaling downstream of NF1 loss-of-function variants / positive regulation of actin cytoskeleton reorganization / SOS-mediated signalling / Activated NTRK3 signals through RAS / Activated NTRK2 signals through RAS / SHC1 events in ERBB4 signaling / positive regulation of protein targeting to membrane / Signalling to RAS / SHC-related events triggered by IGF1R / Activated NTRK2 signals through FRS2 and FRS3 / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / adipose tissue development / Estrogen-stimulated signaling through PRKCZ / Signaling by PDGFRA extracellular domain mutants / Signaling by PDGFRA transmembrane, juxtamembrane and kinase domain mutants / SHC-mediated cascade:FGFR3 / MET activates RAS signaling / Signaling by FGFR4 in disease / positive regulation of phospholipase C activity / SHC-mediated cascade:FGFR2 / Erythropoietin activates RAS / SHC-mediated cascade:FGFR4 / Signaling by FLT3 ITD and TKD mutants / SHC-mediated cascade:FGFR1 / FRS-mediated FGFR3 signaling / Tie2 Signaling / Signaling by FGFR3 in disease / FRS-mediated FGFR2 signaling / FRS-mediated FGFR4 signaling / EPHB-mediated forward signaling / FRS-mediated FGFR1 signaling / G protein activity / Signaling by FGFR2 in disease / GRB2 events in EGFR signaling / Ras activation upon Ca2+ influx through NMDA receptor / SHC1 events in EGFR signaling / p38MAPK events / Signaling by FLT3 fusion proteins / FLT3 Signaling / EGFR Transactivation by Gastrin / small monomeric GTPase / GRB2 events in ERBB2 signaling / Signaling by FGFR1 in disease / intrinsic apoptotic signaling pathway / CD209 (DC-SIGN) signaling / SHC1 events in ERBB2 signaling / Downstream signal transduction / NCAM signaling for neurite out-growth / Insulin receptor signalling cascade / Constitutive Signaling by Overexpressed ERBB2 / Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants / VEGFR2 mediated cell proliferation / positive regulation of epithelial cell proliferation / regulation of long-term neuronal synaptic plasticity / positive regulation of JNK cascade / RAF activation / FCERI mediated MAPK activation / Signaling by ERBB2 TMD/JMD mutants / animal organ morphogenesis / Signaling by high-kinase activity BRAF mutants / Constitutive Signaling by EGFRvIII / Signaling by ERBB2 ECD mutants / MAP2K and MAPK activation / Signaling by ERBB2 KD Mutants / Signaling by SCF-KIT / cellular response to gamma radiation / Regulation of RAS by GAPs / GDP binding / Negative regulation of MAPK pathway / RAS processing / fibroblast proliferation / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / endocytosis / positive regulation of GTPase activity / chemotaxis / Signaling by BRAF and RAF1 fusions / cellular senescence / positive regulation of fibroblast proliferation / positive regulation of type II interferon production / MAPK cascade / positive regulation of MAP kinase activity / Constitutive Signaling by Ligand-Responsive EGFR Cancer Variants / DAP12 signaling / regulation of cell population proliferation / T cell receptor signaling pathway
Similarity search - Function
Small GTPase, Ras-type / small GTPase Ras family profile. / Rho (Ras homology) subfamily of Ras-like small GTPases / Small GTPase / Ras family / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GTPase HRas
Similarity search - Component
|Biological species||Homo sapiens (human)|
|Method||X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å|
|Authors||Buhrman, G. / Wink, G. / Mattos, C.|
|Citation||Journal: Structure / Year: 2007|
Title: Transformation Efficiency of RasQ61 Mutants Linked to Structural Features of the Switch Regions in the Presence of Raf.
Authors: Buhrman, G. / Wink, G. / Mattos, C.
|Structure viewer||Molecule: |
Downloads & links
A: GTPase HRas
|Components on special symmetry positions|
|#1: Protein|| |
Mass: 18846.193 Da / Num. of mol.: 1 / Fragment: Residues 1-166 / Mutation: Q61V
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HRAS, HRAS1 / Plasmid: pET21 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (unknown) / Strain (production host): BL21 / References: UniProt: P01112
|#2: Chemical||#3: Chemical|| ChemComp-CA / ||#4: Chemical|| ChemComp-GNP / ||#5: Water|| ChemComp-HOH / |
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 2.65 Å3/Da / Density % sol: 53.57 %|
|Crystal grow||Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 |
Details: 20 % PEG 3350, 0.2 M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|Diffraction||Mean temperature: 100 K|
|Diffraction source||Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å|
|Detector||Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Dec 13, 2006 / Details: Mirrors|
|Radiation||Monochromator: Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 1 Å / Relative weight: 1|
|Reflection||Resolution: 1.6→36.8 Å / Num. all: 26159 / Num. obs: 26159 / % possible obs: 98.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Biso Wilson estimate: 21.6 Å2 / Rsym value: 0.037|
|Refinement||Method to determine structure: MOLECULAR REPLACEMENT|
Starting model: PDB entry 1CTQ
Resolution: 1.6→28.28 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 199034.59 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
|Solvent computation||Solvent model: FLAT MODEL / Bsol: 60.5864 Å2 / ksol: 0.351179 e/Å3|
|Displacement parameters||Biso mean: 26.2 Å2|
|Refinement step||Cycle: LAST / Resolution: 1.6→28.28 Å|
|Refine LS restraints|
|LS refinement shell||Resolution: 1.6→1.7 Å / Rfactor Rfree error: 0.012 / Total num. of bins used: 6 |
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