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Open data
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Basic information
Entry | Database: PDB / ID: 2rcj | ||||||
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Title | Solution structure of human Immunoglobulin M | ||||||
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![]() | IMMUNE SYSTEM / polymeric antibodies / immunology / constrained modelling | ||||||
Function / homology | ![]() Fc-gamma receptor I complex binding / complement-dependent cytotoxicity / IgG immunoglobulin complex / antibody-dependent cellular cytotoxicity / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / immunoglobulin complex, circulating / immunoglobulin receptor binding ...Fc-gamma receptor I complex binding / complement-dependent cytotoxicity / IgG immunoglobulin complex / antibody-dependent cellular cytotoxicity / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / immunoglobulin complex, circulating / immunoglobulin receptor binding / FCGR3A-mediated IL10 synthesis / complement activation, classical pathway / Regulation of Complement cascade / FCGR3A-mediated phagocytosis / antigen binding / B cell receptor signaling pathway / Regulation of actin dynamics for phagocytic cup formation / antibacterial humoral response / Interleukin-4 and Interleukin-13 signaling / adaptive immune response / blood microparticle / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Perkins, S.J. / Nealis, A.S. / Sutton, B.J. / Feinstein, A. | ||||||
![]() | ![]() Title: Solution structure of human and mouse immunoglobulin M by synchrotron X-ray scattering and molecular graphics modelling. A possible mechanism for complement activation. Authors: Perkins, S.J. / Nealis, A.S. / Sutton, B.J. / Feinstein, A. #1: ![]() Title: Implications of the near-planar solution structure of human myeloma dimeric IgA1 for mucosal immunity and IgA nephropathy Authors: Bonner, A. / Furtado, P.B. / Almogren, A. / Kerr, M.A. / Perkins, S.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 216.9 KB | Display | ![]() |
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PDB format | ![]() | 138.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 311.3 KB | Display | ![]() |
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Full document | ![]() | 315.1 KB | Display | |
Data in XML | ![]() | 2.2 KB | Display | |
Data in CIF | ![]() | 68.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Details | This structure is a pentamer structure with 4 chains in each subunit - plus an additional J Chain to make it 21 polypeptide chains. |
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Components
#1: Antibody | Mass: 23165.770 Da / Num. of mol.: 10 / Source method: isolated from a natural source Details: Human immunoglobulin M was purified from the serum of a patient with Waldenstrom's disease Source: (natural) ![]() #2: Antibody | Mass: 56759.504 Da / Num. of mol.: 10 / Source method: isolated from a natural source Details: Human immunoglobulin M was purified from the serum of a patient with Waldenstrom's disease Source: (natural) ![]() #3: Protein | | Mass: 10700.027 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Human immunoglobulin M was purified from the serum of a patient with Waldenstrom's disease Source: (natural) ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 | ||||||||||||||||||
Soln scatter | Type: x-ray / Buffer name: 200 MM NACL 12 MM NAHPO4 0.05% NA AZIDE / Conc. range: 2-30 / Data analysis software list: SCTPL2 / Data reduction software list: SWANAL / Detector type: LINEAR DETECTOR / Max mean cross sectional radii gyration: 1.79 nm / Max mean cross sectional radii gyration esd: 0.12 nm / Mean guiner radius: 12.17 nm / Mean guiner radius esd: 0.34 nm / Min mean cross sectional radii gyration: 6.06 nm / Min mean cross sectional radii gyration esd: 0.19 nm / Num. of time frames: 1 / Sample pH: 7 / Source beamline: 7.3 / Source class: Y / Source type: SRS DARESBURY / Temperature: 293 K |
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Processing
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Refinement step | Cycle: LAST
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Soln scatter model | Details: THE DEPOSITED STRUCTURE CONTAINS ONLY CA ATOMS. THE IGM COORDINATES ARE PROVIDED FROM AN EARLY STUDY IN WHICH A MANUAL FIT METHOD BASED ON KNOWN CRYSTAL STRUCTURES FOR IGG WAS USED TO ...Details: THE DEPOSITED STRUCTURE CONTAINS ONLY CA ATOMS. THE IGM COORDINATES ARE PROVIDED FROM AN EARLY STUDY IN WHICH A MANUAL FIT METHOD BASED ON KNOWN CRYSTAL STRUCTURES FOR IGG WAS USED TO DETERMINE STRUCTURES FOR INTACT IGM AND ITS FAB, FAB'2, FC5 AND IGM-S MONOMER SUBUNITS. THESE CRYSTAL STRUCTURES ORIGINATED FROM HUMAN FAB KOL (PDB CODE 2FB4) AND HUMAN FC (PDB CODE 1FC1). THIS PROCEDURE IS APPROXIMATE AND THE IGM MODEL IS NOT CONSIDERED TO BE OPTIMAL OR REFINED AGAINST THE X-RAY SCATTERING DATA. Num. of conformers submitted: 1 / Software list: FRODO, INSIGHT, SCTPL2 |