温度: 285 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7 詳細: 1 microliter of VP1 at 10 mg/ml in 25 mM Na-HEPES, pH 7.8, 100 mM NaCl mixed with 2 microliter of crystallization buffer [25 mM Na-MES, pH 6.5, 1.5% (w/v) PEG 3350] and allowing the drop to ...詳細: 1 microliter of VP1 at 10 mg/ml in 25 mM Na-HEPES, pH 7.8, 100 mM NaCl mixed with 2 microliter of crystallization buffer [25 mM Na-MES, pH 6.5, 1.5% (w/v) PEG 3350] and allowing the drop to equilibrate at 12 C by hanging-drop vapor diffusion with a well solution identical in composition to the drop except for the protein. With micro-seeding, thin, plate-like crystals appeared after 1 day and grew to full size in about two weeks., pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K
モノクロメーター: Double crystal, Si(111) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.97949 Å / 相対比: 1
Reflection
冗長度: 3.9 % / Av σ(I) over netI: 15.7 / 数: 108692 / Rmerge(I) obs: 0.055 / Χ2: 1.04 / D res high: 2.9 Å / D res low: 30 Å / Num. obs: 28000 / % possible obs: 99.8
Diffraction reflection shell
最高解像度 (Å)
最低解像度 (Å)
% possible obs (%)
ID
Rmerge(I) obs
Chi squared
Redundancy
6.23
30
99.5
1
0.022
1.03
3.7
4.95
6.23
99.9
1
0.038
1.083
3.9
4.33
4.95
99.9
1
0.031
1.089
4
3.93
4.33
100
1
0.041
1.008
4
3.65
3.93
100
1
0.065
1.018
4
3.44
3.65
99.8
1
0.092
1.06
4
3.27
3.44
99.7
1
0.137
1.062
3.9
3.12
3.27
99.9
1
0.208
0.992
3.8
3
3.12
99.8
1
0.319
1.04
3.8
2.9
3
99.1
1
0.426
1.035
3.7
反射
解像度: 3.24→30 Å / Num. obs: 18356 / % possible obs: 88.9 % / 冗長度: 4.5 % / Rmerge(I) obs: 0.098 / Χ2: 1.002 / Net I/σ(I): 10.1
反射 シェル
解像度 (Å)
冗長度 (%)
Rmerge(I) obs
Num. unique all
Χ2
% possible all
3.24-3.31
3.7
0.404
1732
0.991
85.7
3.31-3.45
4.3
0.294
1795
1.004
88.9
3.45-3.6
4.7
0.235
1852
1.003
91.2
3.6-3.79
4.6
0.173
1848
1.004
90.9
3.79-4.03
4.7
0.137
1835
1.003
90.1
4.03-4.34
4.7
0.104
1846
0.998
90.1
4.34-4.78
4.7
0.084
1844
1
89.1
4.78-5.46
4.7
0.08
1847
1.005
89.4
5.46-6.87
4.6
0.074
1849
1.007
88
6.87-30
4.5
0.039
1908
1.003
86.1
-
位相決定
位相決定
手法: 多重同系置換・異常分散
Phasing set
ID
1
2
3
4
5
6
7
8
9
Phasing dm
手法: Solvent flattening and Histogram matching / 反射: 27884