- PDB-2qu1: Crystal Structure of a Cyclized GFP Variant -
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基本情報
登録情報
データベース: PDB / ID: 2qu1
タイトル
Crystal Structure of a Cyclized GFP Variant
要素
Green fluorescent protein
キーワード
LUMINESCENT PROTEIN / Protein Expression Testing / Pipeline Development / Protein Structure Initiative / PSI / Center for Eukaryotic Structural Genomics / CESG / Chromophore / Luminescence / Photoprotein
機能・相同性
機能・相同性情報
bioluminescence / generation of precursor metabolites and energy 類似検索 - 分子機能
Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / Mainly Beta 類似検索 - ドメイン・相同性
温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: Protein solution (4.95 mg/ml Protein, 0.050 M Sodium chloride, 0.0003 M TCEP, 0.005 M Bis-Tris pH 7.0) mixed in a 1:1 ratio with the Well solution (0.080 M Calcium chloride, 15% PEG 4000, 0.1 ...詳細: Protein solution (4.95 mg/ml Protein, 0.050 M Sodium chloride, 0.0003 M TCEP, 0.005 M Bis-Tris pH 7.0) mixed in a 1:1 ratio with the Well solution (0.080 M Calcium chloride, 15% PEG 4000, 0.1 M HEPPS pH 8.5) and cryoprotected with well solution supplemented with 20% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: 回転陽極 / タイプ: BRUKER AXS MICROSTAR / 波長: 1.5418 Å