BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 1 ... BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 1 CHAIN(S). AUTHORS STATE THAT THE BIOLOGICAL UNIT OF THIS PROTEIN IS UNKNOWN.
温度: 291 K / 手法: microbatch under oil / pH: 7.5 詳細: Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 20 micro-g/ml Subtilisin, 5 mM DTT. Reservoir solution: 16% PEG 3350, 200 mM Ammonium tartrate, MICROBATCH UNDER OIL, temperature 291K
解像度: 2.4→2.49 Å / 冗長度: 8.1 % / Rmerge(I) obs: 0.391 / Mean I/σ(I) obs: 4.09 / Rsym value: 0.295 / % possible all: 100
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解析
ソフトウェア
名称
バージョン
分類
CNS
1.1
精密化
ADSC
Quantum
データ収集
HKL-2000
データ削減
HKL-2000
データスケーリング
SnB
位相決定
RESOLVE
位相決定
精密化
構造決定の手法: 単波長異常分散 / 解像度: 2.4→29.99 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 136724.33 / Data cutoff low absF: 0 / Isotropic thermal model: OVERALL / 交差検証法: THROUGHOUT / σ(F): 2 / 立体化学のターゲット値: Engh & Huber 詳細: THE FRIEDEL PAIRS WERE USED FOR PHASING. XtalView program has also been used in refinement