- PDB-2q69: Crystal Structure of Nak channel D66N mutant -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2q69
Title
Crystal Structure of Nak channel D66N mutant
Components
Potassium channel protein
Keywords
METAL TRANSPORT / MEMBRANE PROTEIN / INVERTED TEEPEE / HELIX BUNDLE / TETRAMER / CENTRAL CAVITY / ION BINDING
Function / homology
Function and homology information
stabilization of membrane potential / potassium ion leak channel activity / outward rectifier potassium channel activity / identical protein binding / membrane / metal ion binding Similarity search - Function
The biological assembly is a tetramer generated from the dimer in the asymmetric unit by the operations: ROTATION MATRIX: 1.00000 -0.00001 -0.00001 -0.00001 -1.00000 -0.00001 -0.00001 0.00001 -1.00000 TRANSLATION VECTOR IN AS 0.00176 85.48581 129.57211
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Components
#1: Protein
Potassiumchannelprotein
Mass: 12828.309 Da / Num. of mol.: 2 / Mutation: D66N Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus cereus (bacteria) / Plasmid: pQE60 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-blue / References: UniProt: Q81HW2
Resolution: 2.4→50 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: Authors state that some electron density that belongs to lipid molecules is not continuous and was modeled as water molecules for convenience.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.267
930
5 %
RANDOM
Rwork
0.24
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-
-
obs
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18325
99.3 %
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Solvent computation
Bsol: 84.87 Å2
Displacement parameters
Biso mean: 69.429 Å2
Baniso -1
Baniso -2
Baniso -3
1-
2.243 Å2
0 Å2
0 Å2
2-
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5.052 Å2
0 Å2
3-
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-7.295 Å2
Refinement step
Cycle: LAST / Resolution: 2.4→50 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
1646
0
3
34
1683
Refine LS restraints
Refine-ID
Type
Dev ideal
X-RAY DIFFRACTION
c_bond_d
0.007
X-RAY DIFFRACTION
c_angle_d
0.97
Xplor file
Refine-ID
Serial no
Param file
X-RAY DIFFRACTION
1
protein_rep.param
X-RAY DIFFRACTION
2
ion.param
X-RAY DIFFRACTION
3
water_rep.param
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