Crystal Structure of Nak channel D66A, S70E double mutants
Components
Potassium channel protein
Keywords
METAL TRANSPORT / MEMBRANE PROTEIN / INVERTED TEEPEE / HELIX BUNDLE / TETRAMER / CENTRAL CAVITY / ION BINDING
Function / homology
Function and homology information
stabilization of membrane potential / potassium ion leak channel activity / outward rectifier potassium channel activity / identical protein binding / membrane / metal ion binding Similarity search - Function
Resolution: 2.5→50 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: Authors state that some electron density that belongs to lipid molecules is not continuous and was modeled as water molecules for convenience.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.262
790
4.9 %
RANDOM
Rwork
0.241
-
-
-
obs
-
15573
96.1 %
-
Solvent computation
Bsol: 75.703 Å2
Displacement parameters
Biso mean: 66.926 Å2
Baniso -1
Baniso -2
Baniso -3
1-
5.319 Å2
0 Å2
0 Å2
2-
-
1.912 Å2
0 Å2
3-
-
-
-7.231 Å2
Refinement step
Cycle: LAST / Resolution: 2.5→50 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
1646
0
5
17
1668
Refine LS restraints
Refine-ID
Type
Dev ideal
X-RAY DIFFRACTION
c_bond_d
0.007
X-RAY DIFFRACTION
c_angle_d
1.086
Xplor file
Refine-ID
Serial no
Param file
X-RAY DIFFRACTION
1
protein_rep.param
X-RAY DIFFRACTION
2
ion.param
X-RAY DIFFRACTION
3
water_rep.param
+
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