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- PDB-2ou8: Structure of Spin-labeled T4 Lysozyme Mutant T115R1 at Room Tempe... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2ou8 | ||||||
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Title | Structure of Spin-labeled T4 Lysozyme Mutant T115R1 at Room Temperature | ||||||
![]() | Lysozyme | ||||||
![]() | HYDROLASE / Nitroxide / Spin label / T4 lysozyme / Electron paramagnetic resonance / EPR | ||||||
Function / homology | ![]() viral release from host cell by cytolysis / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / host cell cytoplasm / defense response to bacterium Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Guo, Z. / Cascio, D. / Hideg, K. / Hubbell, W.L. | ||||||
![]() | ![]() Title: Structural determinants of nitroxide motion in spin-labeled proteins: Tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme. Authors: Guo, Z. / Cascio, D. / Hideg, K. / Kalai, T. / Hubbell, W.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.1 KB | Display | ![]() |
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PDB format | ![]() | 34.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1009.3 KB | Display | ![]() |
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Full document | ![]() | 1013.9 KB | Display | |
Data in XML | ![]() | 10.7 KB | Display | |
Data in CIF | ![]() | 14.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2igcC ![]() 2ntgC ![]() 2nthC ![]() 2ou9C ![]() 3lzmS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18630.402 Da / Num. of mol.: 1 / Mutation: C54T, C97A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-MTN / | ||
#3: Chemical | ChemComp-BME / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment |
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Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.61 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 6.9 Details: 1.8 M Na/K Phosphate, 240 mM NaCl, 40 mM 2-hydroxyethyl disulfide, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: May 17, 2002 / Details: Osmic-Confocal |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→80 Å / Num. all: 19612 / Num. obs: 19612 / % possible obs: 98.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Rsym value: 0.079 / Χ2: 1.011 / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.8→1.86 Å / Rmerge(I) obs: 0.35 / Mean I/σ(I) obs: 2.8 / Num. unique all: 1908 / Rsym value: 0.35 / Χ2: 0.619 / % possible all: 98.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3LZM Resolution: 1.8→10 Å / Num. parameters: 5743 / Num. restraintsaints: 5343 / Cross valid method: FREE R / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: MOEWS & KRETSINGER, J.MOL.BIOL.91(1973)201-228 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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