ジャーナル: Current Topics in Membranes / 年: 2007 タイトル: Structures of the Prokaryotic Mechanosensitive Channels MscL and MscS 著者: Steinbacher, S. / Bass, R. / Strop, P. / Rees, D.C.
SEQUENCE All the non-histidine residues in the N-terminal 22 residues are part of a cleavable his- ...SEQUENCE All the non-histidine residues in the N-terminal 22 residues are part of a cleavable his-tag construct that was added to the MscL sequence. The protein sequence is not cleaved
手法: 蒸気拡散法, シッティングドロップ法 / pH: 3.7 詳細: PROTEIN CONCENTRATION 15-20 MG/ML AND 0.05% DODECYLMALTOSIDE.PROTEIN WAS MIXED IN A RATIO OF 4:3 OR 3:2 WITH THE RESERVOIR SOLUTION CONTAINING 100-120 mM AMMONIUM SULFUATE, 23-27% TRIETHYLENE ...詳細: PROTEIN CONCENTRATION 15-20 MG/ML AND 0.05% DODECYLMALTOSIDE.PROTEIN WAS MIXED IN A RATIO OF 4:3 OR 3:2 WITH THE RESERVOIR SOLUTION CONTAINING 100-120 mM AMMONIUM SULFUATE, 23-27% TRIETHYLENE GLYCOL, 100 mM GLYCINE, WITH 1-3 mM GD(CL)3 OR SM(CL)3 AND D2O AS THE SOLVENT. 1 mM (NA3)AU(S2O3)2 WAS SOAKED INTO THE CRYSTAL, pH 3.7, VAPOR DIFFUSION, SITTING DROP