Journal: Current Topics in Membranes / Year: 2007 Title: Structures of the Prokaryotic Mechanosensitive Channels MscL and MscS Authors: Steinbacher, S. / Bass, R. / Strop, P. / Rees, D.C.
#1: Journal: Science / Year: 1998 Title: Structure of the MscL homolog from Mycobacterium tuberculosis: A gated mechanosensitive ion channel Authors: Chang, G. / Spencer, R.H. / Lee, A.T. / Barclay, M.T. / Rees, D.C.
SEQUENCE All the non-histidine residues in the N-terminal 22 residues are part of a cleavable his- ...SEQUENCE All the non-histidine residues in the N-terminal 22 residues are part of a cleavable his-tag construct that was added to the MscL sequence. The protein sequence is not cleaved
Mass: 196.967 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Au
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 5.9 Å3/Da / Density % sol: 78.97 %
Crystal grow
Method: vapor diffusion, sitting drop / pH: 3.7 Details: PROTEIN CONCENTRATION 15-20 MG/ML AND 0.05% DODECYLMALTOSIDE.PROTEIN WAS MIXED IN A RATIO OF 4:3 OR 3:2 WITH THE RESERVOIR SOLUTION CONTAINING 100-120 mM AMMONIUM SULFUATE, 23-27% ...Details: PROTEIN CONCENTRATION 15-20 MG/ML AND 0.05% DODECYLMALTOSIDE.PROTEIN WAS MIXED IN A RATIO OF 4:3 OR 3:2 WITH THE RESERVOIR SOLUTION CONTAINING 100-120 mM AMMONIUM SULFUATE, 23-27% TRIETHYLENE GLYCOL, 100 mM GLYCINE, WITH 1-3 mM GD(CL)3 OR SM(CL)3 AND D2O AS THE SOLVENT. 1 mM (NA3)AU(S2O3)2 WAS SOAKED INTO THE CRYSTAL, pH 3.7, VAPOR DIFFUSION, SITTING DROP
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